Morphometric Research Laboratory, North Sichuan Medical College, Nanchong, China.
Andrologia. 2011 Apr;43(2):129-38. doi: 10.1111/j.1439-0272.2009.01034.x. Epub 2010 Dec 29.
Recent study in rabbits demonstrated that vasectomy via the inguinal canal did not result in any spermatogenic damage 3 months postoperation; this study aimed to determine whether the damage would occur in a longer term. The left or right vas deferens was ligated near the epididymal head (unilateral proximal vasectomy, 12 animals) or via the inguinal canal (unilateral distal vasectomy, 11 animals) in adult male rabbits, with a sham operation being performed on the contralateral side. Six months postoperation, testes, epididymides and vasa deferentia were removed and methacrylate resin-embedded sections prepared to evaluate spermatogenesis by histological (qualitative) and stereological (quantitative) studies. The juxta-epididymal segment of the occluded vas deferens was severely distended (filled with sperm) in 10 of the 11 animals with distal vasectomy and moderately or slightly distended in nine of the 12 animals with proximal vasectomy. Severe spermatogenic damage occurred in seven animals with proximal vasectomy (the juxta-epididymal vas moderately or slightly distended), in only one animal with distal vasectomy (the vas not severely distended). In conclusion, spermatogenic damage occurred at 6 months postvasectomy in some animals, especially those with proximal vasectomy and therefore shorter occluded reproductive tract for sperm storage; the damage was probably intra-tract pressure mediated.
最近在兔子身上进行的一项研究表明，经腹股沟管进行输精管切除术 3 个月后不会导致任何生精损伤；本研究旨在确定这种损伤是否会在更长时间内发生。将左侧或右侧输精管在附睾头部附近结扎（12 只动物行单侧近端输精管切除术）或经腹股沟管结扎（11 只动物行单侧远端输精管切除术），对侧行假手术。术后 6 个月，取出睾丸、附睾和输精管，用甲基丙烯酸树脂包埋切片，通过组织学（定性）和体视学（定量）研究评估生精情况。11 只远端输精管结扎动物中有 10 只的闭塞输精管近附睾段严重扩张（充满精子），12 只近端输精管结扎动物中有 9 只中度或轻度扩张。7 只近端输精管结扎动物发生严重生精损伤（近附睾段输精管中度或轻度扩张），仅 1 只远端输精管结扎动物发生严重生精损伤（输精管未严重扩张）。结论：输精管结扎后 6 个月，一些动物（尤其是近端输精管结扎且因此精子储存的闭塞生殖道较短的动物）发生生精损伤；这种损伤可能是由管内压力介导的。
