冠状病毒茎环 2（SL2）的溶液结构揭示了一个典型的 CUYG 四核苷酸环折叠。
The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold.
机构信息
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
出版信息
FEBS Lett. 2011 Apr 6;585(7):1049-53. doi: 10.1016/j.febslet.2011.03.002. Epub 2011 Mar 4.
Abstract
The transcription and replication of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is regulated by specific viral genome sequences within 5'- and 3'-untranslated regions (5'-UTR and 3'-UTR). Here we report the solution structure of 5'-UTR derived stem-loop 2 (SL2) of SARS-CoV determined by NMR spectroscopy. The highly conserved pentaloop of SL2 is stacked on 5-bp stem and adopts a canonical CUYG tetraloop fold with the 3' nucleotide (U51) flipped out of the stack. The significance of this structure in the context of a previous mutagenesis analysis of SL2 function in replication of the related group 2 coronavirus, mouse hepatitis virus, is discussed.
摘要
严重急性呼吸系统综合征（SARS）冠状病毒（SARS-CoV）的转录和复制受到 5'-和 3'-非翻译区（5'-UTR 和 3'-UTR）内特定病毒基因组序列的调节。在这里，我们通过 NMR 光谱法报告了 SARS-CoV 5'-UTR 衍生茎环 2（SL2）的溶液结构。SL2 的高度保守的 pentaloop 堆叠在 5bp 茎上，并采用典型的 CUYG 四loop 折叠，其中 3'核苷酸（U51）翻转出堆叠。讨论了该结构在之前的 SL2 功能突变分析中在相关的第 2 组冠状病毒（鼠肝炎病毒）复制中的意义。
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