National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada R3E 3M4.
Vaccine. 2011 Apr 12;29(17):3118-23. doi: 10.1016/j.vaccine.2011.02.069. Epub 2011 Mar 5.
Influenza virus infections continue to cause production losses in the agricultural industry in addition to being a human public health concern. The primary method to control influenza is through vaccination. However, currently used killed influenza virus vaccines must be closely matched to the challenge virus. The ability of an elastase-dependent live attenuated influenza A virus was evaluated to protect pigs against the pandemic H1N1 2009 influenza virus. Pigs vaccinated intranasally or intratracheally with the elastase-dependent swine influenza virus (SIV) vaccine had significantly reduced macroscopic and microscopic lung lesions and lower viral loads in the lung and in nasal swabs. Thus, elastase-dependent SIV mutants can be used as live-virus vaccines against swine influenza in pigs. In addition, low levels of cross-neutralizing antibodies to H1N1 2009 were elicited prior to challenge by the swine adapted H1N1 avian strain vaccine.
流感病毒感染除了对人类公共卫生造成关注外，还会导致农业产业的生产损失。控制流感的主要方法是通过疫苗接种。然而，目前使用的灭活流感病毒疫苗必须与挑战病毒密切匹配。评估了依赖弹性蛋白酶的减毒活流感 A 病毒在保护猪免受大流行性 H1N1 2009 流感病毒方面的作用。通过鼻腔内或气管内接种依赖弹性蛋白酶的猪流感病毒（SIV）疫苗的猪，其肺部和鼻腔拭子中的宏观和微观肺部病变以及病毒载量显著降低。因此，依赖弹性蛋白酶的 SIV 突变体可用作针对猪流感的活病毒疫苗。此外，通过猪适应的 H1N1 禽流感株疫苗预先接种，引发了针对 H1N1 2009 的低水平交叉中和抗体。
