Kai Shigemi, Akaboshi Takeshi, Waki Masumi, Fuzimaki Teruhisa, Kanazawa Hideko
Kanagawa Prefectural Institute of Public Health, Chigasaki-shi, Kanagawa, Japan.
Shokuhin Eiseigaku Zasshi. 2011;52(1):28-33. doi: 10.3358/shokueishi.52.28.
A method for simultaneous determination of metribuzin (MET) and three metribuzin metabolites in livestock products and seafoods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. MET and its metabolites were extracted from a sample with acetonitrile, followed by InertSepC18 and BondElut SAX cartridge cleanup. The LC separation was performed on a C18 column using 0.01 mol/L ammonium formate-acetonitrile-methanol (70 :21 : 9) as the mobile phase and MS detection with both positive and negative ion electrospray ionization. The mean recoveries from 10 livestock products and seafoods were generally >60%, and the relative standard deviations were <20%
建立了一种液相色谱-串联质谱法（LC-MS/MS）同时测定畜产品和海产品中嗪草酮（MET）及其3种代谢物的方法。MET及其代谢物用乙腈从样品中提取，然后经InertSepC18和BondElut SAX柱净化。液相色谱分离在C18柱上进行，以0.01 mol/L甲酸铵-乙腈-甲醇（70:21:9）为流动相，采用正、负离子电喷雾电离进行质谱检测。10种畜产品和海产品的平均回收率一般>60%，相对标准偏差<20%。
