[婴儿体内乳酸杆菌的种类组成及抗生素耐药性]

[Species composition and antibiotic resistance of lactobacillus in infants].

作者信息

Nikolaeva I V, Shabanova N A, Bondarenko V M, Konovalova G N, Anokhin V A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2010 Nov-Dec(6):70-5.

PMID:21384590

Abstract

AIM

To study species composition and antibiotic resistance of indigenous bacteria from Lactobacillus genus in infants.

MATERIALS AND METHODS

Twenty-six strains of lactobacilli isolated from feces of infants living in Kazan. Species membership of lactobacilli was determined using PCR with primers for identification of 7 species of lactobacilli: L. acidophilus, L. plantarum, L. rhamnosus, L. delbrueckii, L. casei, L. paracasei, and L. zeae. Susceptibility to antibiotics was determined by indicator paper discs method. RESULTS. In breastfed infants L. fermentum and L. rhamnosus dominated, whereas in artificially fed infants colonization with L. casei and L. paracasei was characteristic. L.acidophilus, L. plantarum, and L. zeae were not detected in infants. In 6 cases isolates could be identified only to genus characteristic. Lactobacilli were polyresistant and had from 8 to 15 markers of resistance. All strains were resistant to ciprofloxacin, co-trimoxazole, ceftriaxone, nitroxoline, metronidazole, furazolidone, and 95% of strains were resistant to vancomycin. In 60-70% of cases lactobacilli were susceptible to penicillin, linezolid, erythromycin and lyncomycin.

CONCLUSION

Regional features of species composition and antibiotic resistance of lactobacilli in infants depending on type of feeding were revealed.

摘要

目的

研究婴儿体内乳酸杆菌属本土细菌的物种组成及抗生素耐药性。

材料与方法

从喀山地区婴儿粪便中分离出26株乳酸杆菌。使用针对7种乳酸杆菌（嗜酸乳杆菌、植物乳杆菌、鼠李糖乳杆菌、德氏乳杆菌、干酪乳杆菌、副干酪乳杆菌和玉米乳杆菌）鉴定的引物进行PCR，以确定乳酸杆菌的物种归属。采用纸片扩散法测定对抗生素的敏感性。结果。母乳喂养婴儿中发酵乳杆菌和鼠李糖乳杆菌占主导，而人工喂养婴儿中以干酪乳杆菌和副干酪乳杆菌定殖为特征。未在婴儿中检测到嗜酸乳杆菌、植物乳杆菌和玉米乳杆菌。在6例中，分离株仅能鉴定到属特征。乳酸杆菌具有多重耐药性，有8至15个耐药标记。所有菌株对环丙沙星、复方新诺明、头孢曲松、硝羟喹啉、甲硝唑、呋喃唑酮耐药，95%的菌株对万古霉素耐药。在60 - 70%的病例中，乳酸杆菌对青霉素、利奈唑胺、红霉素和林可霉素敏感。