Kostolanska J, Jakus V, Barak L, Stanikova A, Waczulikova I
1st Department of Pediatrics, Faculty of Medicine, Comenius University and University Hospital for Children, Bratislava, Slovakia.
Bratisl Lek Listy. 2010;111(11):578-85.
We tried to investigate whether the AGEs in serum and lipoperoxides (LPO) monitoring were suitable for an early prediction of diabetic complications (DC) development in diabetological practice. We wanted to find whether it is better to divide the file according to the presence of DC or in terms of glycemic compensation in this study.
79 diabetic patients with duration of disease for at least 5 years were divided in respect to DC presence/absence and also to long-time glycemic compensation. HbA1c was measured by LPLC in fair capillary blood, s-AGEs were estimated spectrofluorimetrically and LPO iodimetrically and spectrophotometrically in serum.
HbA1c, s-AGEs and LPO were significantly higher in the group with DC (+DC) vs. controls and also in -DC vs. controls. HbA1c and s-AGEs were significantly higher in +DC vs. patients without DC (-DC). HbA1c, s-AGEs and LPO were significantly higher in patients with poor glycemic compensation (PGC) compared to controls and HbA1c and LPO in patients with good glycemic compensation (GGC) compared to controls. HbA1c and s-AGEs were significantly higher in PGC vs. GGC. In the group of GGC we have found interesting significant correlations of HbA1c with HDL (r=0.451, p<0.05) and with LDL (r=-0.450, p<0.05).
Our findings suggest that the monitoring of s-AGEs in poorly compensated diabetic patients and LPO in all may be very useful to recognize the risk of complications. The dividing of patient file in terms of long time glycemic compensation is more reliable for research of this issue (Tab. 3, Fig. 6, Ref. 41). Full Text in free PDF www.bmj.sk.
我们试图研究在糖尿病临床实践中，血清晚期糖基化终末产物（AGEs）和脂质过氧化物（LPO）监测是否适合早期预测糖尿病并发症（DC）的发生。在本研究中，我们想了解根据DC的存在情况还是血糖代偿情况对病例进行分类更好。
79例病程至少5年的糖尿病患者，根据是否存在DC以及长期血糖代偿情况进行分组。采用液相色谱法（LPLC）检测微量毛细血管血中的糖化血红蛋白（HbA1c），用荧光分光光度法估算血清晚期糖基化终末产物（s-AGEs），用碘量法和分光光度法检测血清中的LPO。
与对照组相比，有DC的组（+DC）以及无DC的组（-DC）中，HbA1c、s-AGEs和LPO均显著升高。与无DC的患者（-DC）相比，+DC组的HbA1c和s-AGEs显著更高。与对照组相比，血糖代偿差（PGC）的患者中HbA1c、s-AGEs和LPO显著更高，与对照组相比，血糖代偿良好（GGC）的患者中HbA1c和LPO显著更高。与GGC组相比，PGC组的HbA1c和s-AGEs显著更高。在GGC组中，我们发现HbA1c与高密度脂蛋白（HDL）（r = 0.451，p < 0.05）以及与低密度脂蛋白（LDL）（r = -0.450，p < 0.05）之间存在有趣的显著相关性。
我们的研究结果表明，监测血糖代偿差的糖尿病患者的s-AGEs以及所有患者的LPO，对于识别并发症风险可能非常有用。就该问题的研究而言，根据长期血糖代偿情况对病例进行分类更可靠（表3，图6，参考文献41）。全文免费PDF版见www.bmj.sk 。
