PLANTA Angewandte Pflanzengenetik und Biotechnologie, Einbeck, Germany.
Mol Plant Microbe Interact. 2011 Jul;24(7):758-72. doi: 10.1094/MPMI-08-10-0189.
Cercospora leaf spot disease, caused by the fungus Cercospora beticola, is the most destructive foliar disease of sugar beet (Beta vulgaris) worldwide. Despite the great agronomical importance of this disease, little is known about its underlying molecular processes. Technical resources are scarce for analyzing this important crop species. We developed a sugar beet microarray with 44,000 oligonucleotides that represent 17,277 cDNAs. During the four stages of C. beticola-B. vulgaris interactions, we profiled the transcriptional responses of three genotypes: susceptible, polygenic partial resistance, and monogenic resistant. Similar genes were induced in all three genotypes during infection but with striking differences in timing. The monogenic resistant genotype displayed strong defense responses at 1 day postinoculation (dpi). The other genotypes displayed defense responses in a later phase (15 dpi) of the infection cycle. The partially resistant genotype displayed a strong defense response in the late phase of the infection cycle. Furthermore, the partially resistant genotype expressed pathogen-related transcripts that the susceptible genotype lacked. These results indicate that resistance was achieved by the ability to mount an early defense response, and partial resistance was determined by additional defense and signaling transcripts that allowed effective defense in the late phase of the infection cycle.
菜豆壳球孢叶斑病，由真菌菜豆壳球孢菌引起，是全球甜菜（Beta vulgaris）最具破坏性的叶斑病。尽管这种疾病具有重要的农业意义，但对其潜在的分子过程知之甚少。分析这种重要作物的技术资源稀缺。我们开发了一种甜菜微阵列，包含 44000 个寡核苷酸，代表 17277 个 cDNA。在菜豆壳球孢菌-甜菜互作的四个阶段中，我们对三种基因型：感病、多基因部分抗性和单基因抗性进行了转录组分析。在感染过程中，所有三种基因型都诱导了相似的基因，但在时间上有显著差异。单基因抗性基因型在接种后 1 天（dpi）表现出强烈的防御反应。其他基因型在感染周期的后期（15dpi）表现出防御反应。部分抗性基因型在感染周期的后期表现出强烈的防御反应。此外，部分抗性基因型表达了感病基因型缺乏的与病原体相关的转录本。这些结果表明，抗性是通过早期防御反应的能力实现的，而部分抗性是由额外的防御和信号转导转录本决定的，这些转录本允许在感染周期的后期进行有效的防御。
