Guimezanes A, Schmitt-Verhulst A M
Centre d'Immunologie INSERM-CNRS, Marseille, France.
Cell Immunol. 1990 Apr 15;127(1):187-98. doi: 10.1016/0008-8749(90)90124-a.
The relationship between the T cell receptor (TcR) for antigen (Ag) and the Lyt-2/3 molecule during T cell activation was studied using the T cell clone KB5.C20, which is dependent upon Lyt-2 for target cell killing. This cytolytic T cell clone can be activated to secrete IFN-gamma by stimulation with H-2Kb expressing cells or with monoclonal antibodies directed against a clonotypic structure of the TcR or against associated CD3 molecules. IFN-gamma production induced by H-2Kb can be inhibited by anti-Lyt-2mAb. In addition, TcR-mediated activation using the anticlonotypic mAb Désiré-1 in soluble form can be inhibited by anti-Lyt-2 mAb in soluble form either as a divalent IgG or as its monovalent Fab fragment. Anti-Lyt-2 mAb immobilized on plastic wells was also inhibitory. Stimulation induced by the anti-TcR mAb or by anti-CD3 mAb immobilized on plastic can be inhibited only with plastic immobilized and not with soluble anti-Lyt-2mAb, however. These results are discussed in terms of local interactions between TcR and Lyt-2 molecules.
利用T细胞克隆KB5.C20研究了T细胞活化过程中抗原（Ag）的T细胞受体（TcR）与Lyt-2/3分子之间的关系，该克隆依赖Lyt-2进行靶细胞杀伤。通过用表达H-2Kb的细胞或针对TcR克隆型结构或相关CD3分子的单克隆抗体刺激，可激活这种细胞毒性T细胞克隆以分泌γ干扰素（IFN-γ）。抗Lyt-2单克隆抗体可抑制H-2Kb诱导的IFN-γ产生。此外，使用可溶性抗独特型单克隆抗体Désiré-1介导的TcR激活可被可溶性抗Lyt-2单克隆抗体以二价IgG或其单价Fab片段的形式抑制。固定在塑料孔上的抗Lyt-2单克隆抗体也具有抑制作用。然而，固定在塑料上的抗TcR单克隆抗体或抗CD3单克隆抗体诱导的刺激只能被固定在塑料上的而不是可溶性抗Lyt-2单克隆抗体抑制。根据TcR和Lyt-2分子之间的局部相互作用对这些结果进行了讨论。
