Coméra C, Rothhut B, Russo-Marie F
Institut National de la Santé et de la Recherche Médicale, U129, Paris, France.
Eur J Biochem. 1990 Feb 22;188(1):139-46. doi: 10.1111/j.1432-1033.1990.tb15381.x.
Calcium-dependent phospholipid binding and phospholipase A2 inhibitory proteins were isolated from human mononuclear cells. Lipocortins I and II were present whereas lipocortin IV (endonexin I) was not. The other proteins were purified to homogeneity and shown to have molecular masses of 35, 36, 32 and 73 kDa. The 36-kDa and 73-kDa proteins are related, the smaller appears to be part of the larger. The 73-kDa protein is related to the 67-kDa calelectrin and to lipocortin VI; the 32-kDa protein is different from endonexin I but related to chromobindin 7 and to lipocortin V. The 35-kDa protein has been identified by tryptic peptide sequencing as lipocortin III. All these proteins inhibit phospholipase A2 activity in vitro and the three smaller ones inhibit the [3H]arachidonic acid release from prelabelled monocytes induced by the calcium ionophore A23187 in a dose-dependent manner.
从人单核细胞中分离出钙依赖性磷脂结合蛋白和磷脂酶A2抑制蛋白。其中存在脂皮质素I和II，而脂皮质素IV（内动蛋白I）不存在。其他蛋白质被纯化至同质，并显示其分子量分别为35、36、32和73 kDa。36 kDa和73 kDa的蛋白质相关，较小的蛋白质似乎是较大蛋白质的一部分。73 kDa的蛋白质与67 kDa的钙电蛋白和脂皮质素VI相关；32 kDa的蛋白质与内动蛋白I不同，但与嗜铬结合蛋白7和脂皮质素V相关。通过胰蛋白酶肽测序鉴定出35 kDa的蛋白质为脂皮质素III。所有这些蛋白质在体外均抑制磷脂酶A2活性，并且三个较小的蛋白质以剂量依赖性方式抑制钙离子载体A23187诱导的预标记单核细胞中[3H]花生四烯酸的释放。
