Institut für Biophysik, Universität Bremen, Bremen, Germany.
J Phys Condens Matter. 2010 May 19;22(19):194106. doi: 10.1088/0953-8984/22/19/194106. Epub 2010 Apr 26.
Mouse embryonic fibroblasts explore the chemical suitability before spreading on a given substrate. We find this early phase of cell spreading to be characterized by transient adhesion patches with a typical mean size of (1.0 ± 0.4) µm and a lifetime of (33 ± 12) s. Eventually, these patches fuse to initiate extensive spreading of the cell. We monitor cell adhesion using reflection interference contrast and total internal reflection fluorescence microscopy. Digital time lapse movies are analysed employing spatio-temporal correlation functions of adhesion patterns. Correlation length and time can be scaled to obtain a master curve at the fusion point.
鼠胚成纤维细胞在铺展到特定底物之前会探索化学适宜性。我们发现细胞铺展的早期阶段的特征是具有短暂黏附斑，其典型平均大小为(1.0 ± 0.4) µm，寿命为(33 ± 12) s。最终，这些黏附斑融合以启动细胞的广泛铺展。我们使用反射干扰对比和全内反射荧光显微镜监测细胞黏附。通过黏附模式的时空相关函数分析数字延时电影。相关长度和时间可以进行缩放，以在融合点获得主曲线。
