Shroff K E, Sengupta S R, Kamat R S
Department of Immunology, Haffkine Institute, Parel, Bombay, India.
Int J Lepr Other Mycobact Dis. 1990 Mar;58(1):50-7.
The route of immunization was observed to play a significant role in deciding the outcome of immunization with killed mycobacterial vaccines. Earlier we reported that the slow growers were immunogenic by both the intraperitoneal (i.p.) and intradermal (i.d.) routes. In contrast, the rapid growers were immunogenic by the i.d. route only. Both rapid and slow growers generated the classical, antigen-specific Lyt-2 positive, T-cell-mediated suppression after i.p. immunization but not after i.d. immunization. Thus, in the case of the slow growers, T-cell-mediated suppression was only a component of the immune response generated after i.p. immunization. In contrast, in the case of Mycobacterium vaccae and the other rapid growers, the T-cell-mediated suppression was the predominant response with i.p. immunization. The T-cell-mediated suppression generated by i.p. immunization exhibited crossreactivity, the spectrum of which was dependent upon the dose of the immunization.
免疫途径在决定用灭活分枝杆菌疫苗进行免疫的结果方面起着重要作用。我们之前报道过，生长缓慢的菌株通过腹腔内（i.p.）和皮内（i.d.）途径均具有免疫原性。相比之下，生长快速的菌株仅通过皮内途径具有免疫原性。生长快速和缓慢的菌株在腹腔内免疫后均产生经典的、抗原特异性Lyt-2阳性的T细胞介导的抑制作用，但在皮内免疫后则不会。因此，对于生长缓慢的菌株，T细胞介导的抑制只是腹腔内免疫后产生的免疫反应的一个组成部分。相比之下，对于母牛分枝杆菌和其他生长快速的菌株，T细胞介导的抑制是腹腔内免疫的主要反应。腹腔内免疫产生的T细胞介导的抑制表现出交叉反应性，其范围取决于免疫剂量。
