Jia Yanyan, Zhang Yan, Wang Chao, Wang Zhirui, Liu Yu, Wang Jingwen, Wen Aidong
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Shaanxi, China.
Biomed Chromatogr. 2011 Oct;25(10):1138-43. doi: 10.1002/bmc.1583. Epub 2011 Mar 9.
A simple, rapid and accurate liquid chromatography-tandem mass spectrometry method has been developed. After a liquid-liquid extraction procedure, samples were chromatographed on an Agilent TC-C(18) (150 × 4.6 mm, 5 μm) column using an isocratic elution mobile phase composed of methanol and distilled water (70:30, v/v) at a flow rate of 0.5 mL/min. After single-dose administration of 0.5, 1 and 2 mg metolazone, the t(1/2) values were 6.6 ± 2.8, 7.9 ± 1.2 and 7.6 ± 1.9 h, respectively. The pharmacokinetic parameters of multiple doses (1 mg metolazone) were as follows: t(1/2) was 8.9 ± 1.0 h; C(max) was 22.4 ± 5.0 ng/mL; and AUC(0-48) was 156.8 ± 31.6 ng h/mL.
已开发出一种简单、快速且准确的液相色谱 - 串联质谱法。经过液 - 液萃取程序后，样品在安捷伦TC - C(18)（150×4.6 mm，5μm）柱上进行色谱分析，使用由甲醇和蒸馏水（70:30，v/v）组成的等度洗脱流动相，流速为0.5 mL/min。单次给予0.5、1和2 mg美托拉宗后，t(1/2)值分别为6.6±2.8、7.9±1.2和7.6±1.9小时。多次给药（1 mg美托拉宗）的药代动力学参数如下：t(1/2)为8.9±1.0小时；C(max)为22.4±5.0 ng/mL；AUC(0 - 48)为156.8±31.6 ng h/mL。
