采用差分脉冲吸附溶出伏安法对青霉素 G 进行纳摩尔级别的定量分析。
Quantification of sub-nanomolar levels of Penicillin G by differential pulse adsorptive stripping voltammetry.
机构信息
Department of Chemistry, Ilam University, Ilam, Iran.
出版信息
Drug Test Anal. 2012 Feb;4(2):140-4. doi: 10.1002/dta.256. Epub 2011 Mar 9.
A novel selective and sensitive method is developed for determination of Penicillin G by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV). Penicillin G gave well-resolved diffusion-controlled cathodic peaks at -0.42 and -0.584 V, respectively (vs Ag/AgCl) in pH 7.50 of borate buffer. Optimal conditions were obtained as pH 7.50, accumulation potential of -0.2 V (vs Ag/AgCl), accumulation time of 120 s, and scan rate of 100 mV/s. Under the optimized conditions, a linear calibration curve was established for the concentration of Penicillin G in the range of 0.007-2.13 µg/ml with a detection limit of 0.000717 µg/ml. The procedure was successfully applied to the determination of Penicillin G in various medicine and biological samples. The relative standard deviation of the method at 0.05 and 0.5 µg/ml Penicillin G, for 10 runs, was 2.55% and 2.06%, respectively.
一种新的、选择性强且灵敏的方法被开发出来，用于通过差分脉冲吸附溶出伏安法（DPAdSV）测定青霉素 G。在硼酸缓冲液的 pH 7.50 下，青霉素 G 在 -0.42 和 -0.584 V（相对于 Ag/AgCl）处分别给出了良好分离的扩散控制阴极峰。最优条件为 pH 7.50、-0.2 V（相对于 Ag/AgCl）的富集电位、120 s 的富集时间和 100 mV/s 的扫描速率。在优化条件下，青霉素 G 的浓度在 0.007-2.13 µg/ml 范围内建立了线性校准曲线，检测限为 0.000717 µg/ml。该方法成功应用于各种药物和生物样品中青霉素 G 的测定。在 0.05 和 0.5 µg/ml 青霉素 G 时，该方法的相对标准偏差为 2.55%和 2.06%，分别进行了 10 次运行。
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