Ye Li, Wang Shibin
Department of Biological Engineering and Technology, Huaqiao University, Xiamen 361021, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Dec;26(12):1611-7.
For the regenerative therapy of refractory diseases, stem cells have become an excellent source of seed cells due to their strong self-renewal and multi-differentiation abilities. Microcapsules can provide a three-dimensional growth environment with a good immunoisolation and biocompatibility for cells, and the microencapsulation of stem cells provides a new technical support for large-scale cell culture with high activities in vitro and long-term preservation, consequently opening up a new alternative for cell transplantation. In this review, we first outlined the development of microencapsulation, then introduced the present materials and methods for the microencapsulation of stem cells and its immunoisolation, and discussed the progress in microencapsulation technology, various types of stem cell used in recent years in details. Finally, we addressed perspectives of stem cell microencapsulation technology.
对于难治性疾病的再生治疗，干细胞因其强大的自我更新和多分化能力，已成为优良的种子细胞来源。微胶囊可为细胞提供具有良好免疫隔离和生物相容性的三维生长环境，干细胞的微囊化可为体外高活性大规模细胞培养和长期保存提供新的技术支持，从而为细胞移植开辟了新的选择。在本综述中，我们首先概述了微囊化的发展，然后介绍了目前用于干细胞微囊化及其免疫隔离的材料和方法，并详细讨论了微囊化技术的进展、近年来使用的各类干细胞。最后，我们阐述了干细胞微囊化技术的前景。
