Singh Chhug, Soni Ravindra, Jain Sourabh, Roy Subhadip, Goel Reeta
Department of Microbiology, CBSH, G.B. Pant University of Agriculture and Technology, Pantnagar - 263 145, India.
J Environ Biol. 2010 Sep;31(5):553-6.
Six soil samples (Pantnagar, Chamoli, Almora, Ranichauri, Pithoragarh and Badrinath) belonging to different geographical locations of Western Himalayas in India, were analyzed to diversify the nitrogen fixing bacterial community using nifH gene biomarker DNA from soil samples were isolated and amplified using nifH gene specific primers. Genomic DNA and PCR amplified products were then individually subjected to restriction digestion with tetra to octacutter enzymes (AluI, MspI, BgIII, XbaI, HindIII, HaeIII, AluI, MspI and PasI. Further restriction pattern was studied by preparing dendograms on the basis of similarity matrix and compared for the nifH community. It was observed that temperate region soils (Ranichauri and Pithoragarh) were negative for nifH marker while subalpine region (Badrinath) and tarai region soils (Pantnagar) documented similar nifH community. Moreover; the direct genomic DNA restriction analysis indicated that subalpine region soil (Badrinath) was most diversified.
对来自印度西喜马拉雅山脉不同地理位置的六个土壤样本（潘特纳加尔、查莫利、阿尔莫拉、拉尼乔里、皮托拉加尔和巴德里纳特）进行了分析，以利用nifH基因生物标志物使固氮细菌群落多样化。从土壤样本中分离出DNA，并使用nifH基因特异性引物进行扩增。然后，将基因组DNA和PCR扩增产物分别用四到八碱基切割酶（AluI、MspI、BgIII、XbaI、HindIII、HaeIII、AluI、MspI和PasI）进行限制性消化。通过基于相似性矩阵制备树状图进一步研究限制性模式，并对nifH群落进行比较。观察到温带地区土壤（拉尼乔里和皮托拉加尔）的nifH标记为阴性，而亚高山地区（巴德里纳特）和台拉伊地区土壤（潘特纳加尔）记录了相似的nifH群落。此外，直接基因组DNA限制性分析表明亚高山地区土壤（巴德里纳特）最为多样化。
