通过凝胶过滤色谱法纯化噬菌体DNA。

Purification of bacteriophage DNA by gel filtration chromatography.

作者信息

González A, Gómez-Márquez J

机构信息

Departmento de Bioquímica e Bioloxía Molecular, Facultade de Bioloxía, Universidade de Santiago de Compostela, Spain.

出版信息

Genet Anal Tech Appl. 1990 Feb;7(1):2-4. doi: 10.1016/0735-0651(90)90037-g.

DOI:10.1016/0735-0651(90)90037-g

PMID:2138902

Abstract

Two fast and effective methods for high-scale purification of linear phage lambda DNA and circular double-stranded M13 replicative form are presented. A substantial reduction of time is attained by avoiding the long-term CsCl gradient centrifugations and dialysis common to standard procedures. Biologically active DNA preparations, free of chromosomal DNA and RNA, are obtained by including a simple gel filtration chromatography as the last step of purification. Yields are comparable to those from previously described methods.

摘要

本文介绍了两种用于大规模纯化线性噬菌体λDNA和环状双链M13复制型的快速有效方法。通过避免标准程序中常见的长期CsCl梯度离心和透析，可大幅减少时间。通过在纯化的最后一步加入简单的凝胶过滤色谱法，可获得不含染色体DNA和RNA的具有生物活性的DNA制剂。产量与先前描述的方法相当。