Hemmer J
University of Ulm, Department of Oral and Maxillofacial Surgery, F.R.G.
Eur J Cancer. 1990 Feb;26(2):113-5. doi: 10.1016/0277-5379(90)90292-2.
Samples of 33 primary and previously untreated squamous cell carcinomas of the oral cavity were labelled by in vitro bromodeoxyuridine (BURD) incubation. Positive cells were visualized by indirect immunofluorescence antibody labelling. The cells were examined by fluorescence microscopy and the labelling index (LI) was calculated. The values ranged from 0 to 23.2% with a median of 2.6%. The LIs of T3 tumors were significantly higher than those of T1 and T2 carcinomas. The LIs of primary tumors showing cervical lymph node metastases at the time of biopsy also had significantly higher values than tumors without evidence of lymph node involvement. The BURD technique seems to represent a promising tool for studying the clinical implications of the proliferative behavior of human tumors.
对33例原发性且未经治疗的口腔鳞状细胞癌样本进行体外溴脱氧尿苷（BURD）孵育标记。通过间接免疫荧光抗体标记使阳性细胞可视化。用荧光显微镜检查细胞并计算标记指数（LI）。其值范围为0至23.2%，中位数为2.6%。T3期肿瘤的LI显著高于T1和T2期癌。活检时出现颈部淋巴结转移的原发性肿瘤的LI值也显著高于无淋巴结受累证据的肿瘤。BURD技术似乎是研究人类肿瘤增殖行为临床意义的一种有前景的工具。
