Stuber G, Vánky F, Pócsik E, Benczúr M, Klein E
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Cancer Immunol Immunother. 1990;31(2):76-80. doi: 10.1007/BF01742369.
The kinetics of interleukin-2 receptor (IL-2R) expression and the [3H]dT incorporation of blood lymphocytes after the first and the second stimulation with allogeneic leukocytes (primary and secondary MLC) or with the autologous tumor cells (primary and secondary MLTC) were compared. The expression of IL-2R paralleled the induction of DNA synthesis. The proportion of IL-2R+ cells of the unprimed donors peaked earlier in the secondary MLC as compared to the primary MLC (on days 3 and 5 respectively). In MLC of alloimmunized healthy individuals and in the MLTC of cancer patients the highest proportions of IL-2R+ cells were detected between days 2 and 3 after both the first and second stimulations. Thus the first in vitro stimulation in the MLTC showed similar kinetics to those of the secondary MLC of unprimed individuals and to the primary MLC response of the allo-immunized individuals. The findings in the MLTC substantiate the hypothesis that cancer patients can be sensitized to their own tumors. The kinetics of the appearance of the IL-2R together with the characteristics of the IL-2-propagated cultures provide useful information for the strategy of expansion of auto-tumor reactive lymphocyte populations.
比较了用同种异体白细胞（初次和二次混合淋巴细胞培养，MLC）或自体肿瘤细胞（初次和二次肿瘤淋巴细胞培养，MLTC）进行初次和二次刺激后，白细胞介素-2受体（IL-2R）表达的动力学以及血液淋巴细胞中[3H]dT掺入情况。IL-2R的表达与DNA合成的诱导平行。与初次MLC相比，未致敏供体的IL-2R+细胞比例在二次MLC中达到峰值的时间更早（分别在第3天和第5天）。在同种免疫健康个体的MLC以及癌症患者的MLTC中，初次和二次刺激后第2至3天检测到的IL-2R+细胞比例最高。因此，MLTC中的初次体外刺激显示出与未致敏个体的二次MLC以及同种免疫个体的初次MLC反应相似的动力学。MLTC中的发现证实了癌症患者可对自身肿瘤产生致敏作用的假说。IL-2R出现的动力学以及IL-2增殖培养物的特性为自体肿瘤反应性淋巴细胞群体的扩增策略提供了有用信息。
