Lee Dong-Youn, Yang Jun-Mo, Mun Goo-Hyun, Jang Kee-Taek, Cho Kwang-Hyun
Department of Dermatology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Am J Dermatopathol. 2011 May;33(3):266-70. doi: 10.1097/DAD.0b013e3181f45611.
In the frozen longitudinal section of the nail unit, CD10 was previously found in nail mesenchymal cells beneath nail matrix, and we proposed calling the nail mesenchymal cells onychofibroblasts. In this study, to further characterize nail mesenchyme containing onychofibroblasts, we examined the expression of several mesenchymal markers immunohistochemically in transverse paraffin sections of the nail unit. CD10 was strongly expressed in the nail mesenchyme containing onychofibroblasts beneath the nail matrix. However, CD10 was not observed in dermal fibroblasts and surrounding extracellular matrix of the lateral nail fold (LNF), except around blood vessels and eccrine structures. In addition, versican was expressed diffusely in the nail mesenchyme containing onychofibroblasts in contrast to the dermis of LNF. Fibrillin, which is a major component of elastic fiber in the dermis, was expressed very weakly on the nail mesenchyme below the nail matrix but was expressed strongly in the dermis of LNF. These findings support the existence of specialized nail mesenchyme containing onychofibroblasts that is distinguished from the dermis of LNF.
在甲单位的冷冻纵切面上，先前在甲母质下方的甲间充质细胞中发现了CD10，我们提议将这些甲间充质细胞称为甲成纤维细胞。在本研究中，为了进一步表征含有甲成纤维细胞的甲间充质，我们在甲单位的横向石蜡切片上通过免疫组织化学检查了几种间充质标志物的表达。CD10在甲母质下方含有甲成纤维细胞的甲间充质中强烈表达。然而，除了血管和小汗腺结构周围外，在甲侧襞（LNF）的真皮成纤维细胞和周围细胞外基质中未观察到CD10。此外，与LNF的真皮相比，多功能蛋白聚糖在含有甲成纤维细胞的甲间充质中呈弥漫性表达。原纤蛋白是真皮中弹性纤维的主要成分，在甲母质下方的甲间充质中表达非常弱，但在LNF的真皮中表达强烈。这些发现支持存在含有甲成纤维细胞的特殊甲间充质，它与LNF的真皮不同。
