Smith R H, Ulian E, Gould J H
Texas A&M University, College Station, TX.
Methods Mol Biol. 1990;6:335-40. doi: 10.1385/0-89603-161-6:335.
The terms "meristem" and "shoot tip" culture have often been indiscriminately interchanged. According to Cutter 1), the apical meristem refers to only that portion of the shoot apex lying distal to the youngest leaf primordium. The shoot apex, or shoot tip, consists of the apical meristem and one to three subjacent leaf primordia. True apical meristem culture of higher plants was first demonstrated by Smith and Murashige 2). The isolated tissues of the apical domes develop directly into plants, demonstrating the developmental automony of the angiosperm shoot apical meristem.
“分生组织”和“茎尖”培养这两个术语常常被不加区分地互换使用。根据卡特（1）的说法，顶端分生组织仅指茎尖中位于最幼嫩叶原基远端的那部分。茎尖，或称为茎梢，由顶端分生组织和其下方的一至三个叶原基组成。高等植物真正的顶端分生组织培养最早由史密斯和村濑（2）证明。顶端圆顶的分离组织直接发育成植株，这证明了被子植物茎尖分生组织的发育自主性。
