Tisserat B
United States Department of Agriculture, Pasadena, CA.
Methods Mol Biol. 1990;6:563-9. doi: 10.1385/0-89603-161-6:563.
This chapter is concerned with the use of mechanical and electronic techniques (i. e., the Automated Plant Culture System [APB]) to aid in the sterile cultivation of plants for the purpose of prolonging culture life and/ or increasing yields over that obtained from conventional technology (bd1,bd2). The use of machines to facilitate the culturing of plants in vitro is common (e. g., shakers, roller bottle apparatuses, incubators, magnetic stirrers, and roller drums). However, current techniques employed in plant tissue culture (i. e., agar or liquid culture), developed about 30 yr ago, are highly labor intensive because of the requirement for numerous hand-plant interactions in the culturing of plants in a sterile environment. Culture of any plant in vitro requires periodic transferring to fresh medium. Nutrient medium is either exhausted and/or altered by plant cultures, thus requiring the constant transfers to fresh medium (usually every 4-8 wk or sooner). The system presented in this chapter is meant to reduce dependence on labor through substitution of hand-plant interactions with machine-plant interactions via automatic medium replenishment (Fig. 1). The APCS culture vessel is, therefore, large enough to accommodate the increasing plant culture size associated with long-term growing of plants in a single culture vessel.
本章涉及使用机械和电子技术（即自动化植物培养系统[APB]）来辅助植物的无菌培养，目的是延长培养寿命和/或提高产量，使其超过传统技术（bd1，bd2）所获得的产量。使用机器来促进植物的体外培养很常见（例如，振荡器、滚瓶装置、培养箱、磁力搅拌器和滚筒）。然而，大约30年前开发的植物组织培养中使用的当前技术（即琼脂或液体培养），由于在无菌环境中培养植物时需要大量的人工操作，劳动强度很大。任何植物的体外培养都需要定期转移到新鲜培养基中。营养培养基会被植物培养物耗尽和/或改变，因此需要不断转移到新鲜培养基中（通常每4 - 8周或更短时间）。本章介绍的系统旨在通过自动补充培养基，用机器与植物的相互作用替代人工与植物的相互作用，从而减少对人工的依赖（图1）。因此，APCS培养容器足够大，能够容纳与在单个培养容器中长期培养植物相关的不断增加的植物培养规模。
