Talavera A, Rodriguez J M
Centro Nacional de Biotechnologia, Madrid, Spain.
Methods Mol Biol. 1992;8:235-48. doi: 10.1385/0-89603-191-8:235.
In Chapter 20 , a general procedure was described for the construction of the intermediate vectors necessary for the insertion of foreign DNA sequences into the vaccinia virus (Vv) genome. The principles and basic methodology for the isolation of recombinant vaccinia viruses will be discussed in this chapter.
在第20章中，描述了构建将外源DNA序列插入痘苗病毒（Vv）基因组所需的中间载体的一般程序。本章将讨论重组痘苗病毒分离的原理和基本方法。
