Qiao Hongxiang, Tong Yinghui, Han Hongcan, Xu Wei, Ren Zihua, Ouyang Jingfeng, Chen Yan
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Pharmazie. 2011 Jan;66(1):37-43.
Baicalin, isolated from the root of Scutellaria baicalensis Georgi, has shown anti-inflammatory and antioxidant activities, while mesenchymal stem cells (MSCs) have the capability of self-renewal and multilineage differentiation. In the present study, we found that baicalin could promote differentiation of bone marrow-derived MSCs into hepatocytes in vitro. We then compared the therapeutic effects of five therapeutic regimens for hepatic fibrosis induced by carbon tetrachloride in vivo by analysis of serum enzymes, morphological characteristics, cytokines and cell engraftment. Transplantation of MSCs alone was able to promote partial recovery of liver function and suppression of liver inflammation, but showed little effect on reducing the fibrotic area; transplantation with baicalin-treated MSCs gave an improved therapeutic effect; MSC transplantation and baicalin administration showed a synergistic effect; transplantation with baicalin-treated MSCs in combination with baicalin administration had the best therapeutic effect for hepatic fibrosis. Therefore, we conclude that transplantation of pre-differentiated MSC together with baicalin administration may serve as an effective therapeutic regimen for severe liver diseases.
从黄芩（Scutellaria baicalensis Georgi）根中分离得到的黄芩苷已显示出抗炎和抗氧化活性，而间充质干细胞（MSCs）具有自我更新和多向分化的能力。在本研究中，我们发现黄芩苷能够在体外促进骨髓来源的间充质干细胞向肝细胞分化。然后，我们通过分析血清酶、形态学特征、细胞因子和细胞植入情况，比较了五种治疗方案对四氯化碳诱导的体内肝纤维化的治疗效果。单独移植间充质干细胞能够促进肝功能部分恢复并抑制肝脏炎症，但对减少纤维化面积效果甚微；移植经黄芩苷处理的间充质干细胞具有更好的治疗效果；间充质干细胞移植与黄芩苷给药显示出协同作用；移植经黄芩苷处理的间充质干细胞并联合黄芩苷给药对肝纤维化具有最佳治疗效果。因此，我们得出结论，预分化的间充质干细胞移植联合黄芩苷给药可能是治疗严重肝脏疾病的有效治疗方案。
