Peng Linda X, Wallace Morgan, Andaloro Bridget, Fallon Dawn, Fleck Lois, Delduco Dan, Tice George
DuPont Qualicon, Rt. 141 & Henry Clay Rd, Wilmington, DE 19880-0400, USA.
J AOAC Int. 2011 Jan-Feb;94(1):172-8.
The BAX System PCR assay for Salmonella detection in foods was previously validated as AOAC Research Institute (RI) Performance Tested Method (PTM) 100201. New studies were conducted on beef and produce using the same media and protocol currently approved for the BAX System PCR assay for E. coli O157:H7 multiplex (MP). Additionally, soy protein isolate was tested for matrix extension using the U.S. Food and Drug Administration-Bacteriological Analytical Manual (FDA-BAM) enrichment protocols. The studies compared the BAX System method to the U.S. Department of Agriculture culture method for detecting Salmonella in beef and the FDA-BAM culture method for detecting Salmonella in produce and soy protein isolate. Method comparison studies on low-level inoculates showed that the BAX System assay for Salmonella performed as well as or better than the reference method for detecting Salmonella in beef and produce in 8-24 h enrichment when the BAX System E. coli O157:H7 MP media was used, and soy protein isolate in 20 h enrichment with lactose broth followed by 3 h regrowth in brain heart infusion broth. An inclusivity panel of 104 Salmonella strains with diverse serotypes was tested by the BAX System using the proprietary BAX System media and returned all positive results. Ruggedness factors involved in the enrichment phase were also evaluated by testing outside the specified parameters, and none of the factors examined affected the performance of the assay.
用于食品中沙门氏菌检测的BAX系统PCR检测方法先前已被确认为美国官方分析化学家协会（AOAC）研究机构（RI）的性能测试方法（PTM）100201。使用目前已批准用于大肠杆菌O157:H7多重PCR（MP）检测的BAX系统PCR检测方法的相同培养基和方案，对牛肉和农产品进行了新的研究。此外，使用美国食品药品监督管理局细菌学分析手册（FDA-BAM）富集方案对大豆分离蛋白进行了基质扩展测试。这些研究将BAX系统方法与美国农业部用于检测牛肉中沙门氏菌的培养方法以及FDA-BAM用于检测农产品和大豆分离蛋白中沙门氏菌的培养方法进行了比较。对低水平接种物的方法比较研究表明，当使用BAX系统大肠杆菌O157:H7 MP培养基时，用于检测牛肉和农产品中沙门氏菌的BAX系统检测方法在8 - 24小时富集时的表现与参考方法相当或更好；对于大豆分离蛋白，在乳糖肉汤中富集20小时，随后在脑心浸液肉汤中再生长3小时。使用BAX系统专用培养基对104株具有不同血清型的沙门氏菌组成的包容性菌株进行了测试，所有结果均为阳性。还通过在指定参数范围外进行测试，评估了富集阶段涉及的耐用性因素，所检测的因素均未影响检测方法的性能。
