Tang Zhuzhao, Chen Haode, Song Shiping, Fan Chunhai, Zhang Dabing, Wu Aibo
Shanghai Jiao Tong University, School of Life Science and Biotechnology, Bor Luh Food Safety Center, 800 Dongchuan Rd, Shanghai 200240, People's Republic of China.
J AOAC Int. 2011 Jan-Feb;94(1):307-12.
Recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 microg MWCNT and 5 microL enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion, 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 microg/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5-5 microg/L and 73.4 to 118.4% for carbofuran at 1-10 microg/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring.
重组黑腹果蝇乙酰胆碱酯酶（R-DmAChE）、多壁碳纳米管（MWCNTs）和普鲁士蓝已被组合用于开发一种三电极生物传感器，与我们之前的研究相比，该传感器具有更快的响应速度和更高的稳定性。开发了一种新型一次性丝网印刷电极（SPE），用于快速检测有机磷和氨基甲酸酯类农药。经过优化，将10微克MWCNT和5微升由0.2%戊二醛、0.1% Nafion、0.2%牛血清白蛋白、0.1克/升MWCNT和1.5毫微单位R-DmAChE组成的酶固定溶液固定在每个R-DmAChE/MWCNT SPE上。该生物传感器对敌敌畏（DDV）和克百威农药标准品的检测限为0.5微克/升。在不同加标水平下对蔬菜和水样测试了该生物传感器的性能，发现其具有良好的稳定性和灵敏度。在湖泊和海水样品中，敌敌畏加标水平为0.5-5微克/升时，回收率为82.6%至110.5%，克百威加标水平为1-10微克/升时，回收率为73.4%至118.4%，这表明所提出的方法是食品安全和环境监测中快速检测农药残留和污染物的一种替代手段。
