Centre for Green Chemistry, Monash University , Clayton, VIC 3800, Australia.
J Agric Food Chem. 2011 Apr 27;59(8):3539-43. doi: 10.1021/jf104230f. Epub 2011 Mar 10.
Molecularly imprinted solid phase extraction (MISPE) has been employed to isolate and concentrate bioactive polyphenols from peanut press waste. To this end, a molecularly imprinted polymer (MIP) templated with the phytoalexin (E)-resveratrol has been prepared via self-assembly with the functional monomer 4-vinylpyridine (4VP) in a 1:3 molar ratio. Subsequent molecular interrogation of the MIP binding sites demonstrated preferential structural selectivity for (E)-resveratrol with respect to other structurally related naturally occurring compounds. This selectivity was subsequently exploited to achieve substantial sample cleanup of peanut press waste under aqueous conditions with significant enrichment of (E)-resveratrol (>60 fold) requiring minimal sample preparation.
分子印迹固相萃取（MISPE）已被用于从花生压榨废物中分离和浓缩生物活性多酚。为此，通过 4-乙烯基吡啶（4VP）与功能单体以 1:3 的摩尔比自组装，制备了以植物抗毒素（E）-白藜芦醇为模板的分子印迹聚合物（MIP）。随后对 MIP 结合位点进行分子检测，结果表明其对（E）-白藜芦醇具有优先的结构选择性，而对其他结构相关的天然存在的化合物则没有选择性。这种选择性随后被利用，在水相条件下对花生压榨废物进行了大量的样品净化，（E）-白藜芦醇的富集倍数达到 60 倍以上，且所需的样品制备量很少。
