Ahrén B, Gustafson A, Hedner P, Nilsson H
Acta Endocrinol (Copenh). 1978 Dec;89(4):693-700. doi: 10.1530/acta.0.0890693.
Half lobes of mouse thyroid gland were incubated in vitro with TSH. They released cyclic AMP (cAMP) into the medium in amounts depending on the concentration of TSH. The release of cAMP was greatest during the first hour of incubation then it occurred at a lower rate. With an incubation time of 45 min the medium cAMP levels ranged from 43.0 +/- 11.9 pmole per mg tissue protein for controls to 296.5 +/- 29.2 pmole per mg tissue protein with 5 mU of TSH in the medium. The tissue cAMP level reached a maximum after 15--30 min of incubation with TSH, then it gradually decreased towards control level during 4 h of incubation. With 25 min of incubation the tissue cAMP level was 28.5 +/- 8.8 pmole per mg tissue protein for controls compared to 194.3 +/- 27.0 pmole per mg tissue protein with 5 mU TSH in the incubation medium. The release of thyroxine was of the same order during the later part of the 4 h incubation period compared to the first one. The results illustrate the quantitative importance of cAMP release, and the fact that in the later part of the incubaion period the cell content of cAMP was low while the release of thyroxine remained high.
将小鼠甲状腺的半叶在体外与促甲状腺激素（TSH）一起孵育。它们向培养基中释放环磷酸腺苷（cAMP），释放量取决于TSH的浓度。在孵育的第一个小时内，cAMP的释放量最大，随后释放速率降低。孵育45分钟时，培养基中cAMP水平范围为：对照组每毫克组织蛋白43.0±11.9皮摩尔，培养基中含5 mU TSH时为每毫克组织蛋白296.5±29.2皮摩尔。用TSH孵育15 - 30分钟后，组织cAMP水平达到最大值，然后在4小时的孵育过程中逐渐降至对照水平。孵育25分钟时，对照组组织cAMP水平为每毫克组织蛋白28.5±8.8皮摩尔，而孵育培养基中含5 mU TSH时为每毫克组织蛋白194.3±27.0皮摩尔。在4小时孵育期的后半段，甲状腺素的释放量与第一段相比处于同一水平。这些结果说明了cAMP释放的定量重要性，以及在孵育后期cAMP的细胞含量较低而甲状腺素释放仍保持较高水平这一事实。
