Gupta Sandeep Kumar, Thangaraj Kumarasamy, Singh Lalji
Centre for Cellular and Molecular Biology, Hyderabad, India.
J Forensic Sci. 2011 Sep;56(5):1343-5. doi: 10.1111/j.1556-4029.2011.01750.x. Epub 2011 Mar 10.
In this study, we describe a forensic case dealing with the identification of the source of the processed ivory object by DNA analysis. Two pieces of Lord Krishna's idols from a shop were confiscated by an investigating agency of the Indian government and forwarded to us to identify the source of its origin. We succeeded in isolating DNA from both processed ivory idols by using the phenol/chloroform DNA extraction method. The extracted DNA was subjected to PCR amplification using an elephant-specific mitochondrial DNA (mtDNA) D-loop marker. DNA sequence analysis of the amplified fragment of mtDNA D-loop region confirmed that the idols were consistent with Asian elephant with 99% similarity.
在本研究中，我们描述了一个通过DNA分析来鉴定加工象牙制品来源的法医案例。印度政府的一个调查机构从一家商店没收了两件克里希纳勋爵的雕像，并将其送交我们以确定其来源。我们通过使用酚/氯仿DNA提取方法成功地从两件加工过的象牙雕像中分离出了DNA。提取的DNA使用大象特异性线粒体DNA（mtDNA）D-loop标记进行PCR扩增。对mtDNA D-loop区域扩增片段的DNA序列分析证实，这些雕像与亚洲象一致，相似度为99%。
