多重聚合酶链反应和自动毛细管电泳直接同时检测临床标本中的 6 种性传播病原体。
Direct simultaneous detection of 6 sexually transmitted pathogens from clinical specimens by multiplex polymerase chain reaction and auto-capillary electrophoresis.
机构信息
Department of Clinical Microbiology, Chlamydia and Mycoplasma National Center, Rabin Medical Center, Petach Tikva 49100, Israel.
出版信息
Diagn Microbiol Infect Dis. 2011 May;70(1):17-21. doi: 10.1016/j.diagmicrobio.2010.12.001. Epub 2011 Mar 9.
The availability of a reliable and user-friendly method to identify pathogens causing sexually transmitted diseases (STDs) is essential to reduce the complications and spread of infection. In this study, genital/urinary specimens from 113 patients with STDs were simultaneously tested for 6 pathogens using the automated Seeplex® (Seegene, Seoul, Korea) multiplex polymerase chain reaction (PCR)-based STD6B auto-capillary electrophoresis (ACE) system. The results were compared with conventional reference methods, including culture and PCR tests. The sensitivity of STD6B ACE was found to be 100% for Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Neisseria gonorrhoeae, and Trichomonas vaginalis, and 98% for genital Ureoplasma (U. urealyticum and U. parvum). Specificity ranged from 97% to 100%. One pathogen was detected in 51 specimens, and 2 or more pathogens were detected in 24. In conclusion, the multiplex PCR and ACE system is highly sensitive and specific for the rapid, simultaneous detection of STD pathogens directly from a single specimen.
为了降低性传播疾病（STD）的并发症和传播风险，能够可靠且易于使用的病原体鉴定方法至关重要。在本研究中，使用自动化 Seeplex®（Seegene，韩国首尔）基于多重聚合酶链反应（PCR）的 STD6B 自动毛细管电泳（ACE）系统，对 113 例 STD 患者的生殖/泌尿标本同时进行了 6 种病原体的检测。将结果与传统的参考方法（培养和 PCR 检测）进行了比较。STD6B ACE 的灵敏度对沙眼衣原体、人型支原体、生殖支原体、淋病奈瑟菌和阴道毛滴虫均为 100%，对生殖支原体（U. urealyticum 和 U. parvum）为 98%。特异性范围为 97%至 100%。51 份标本中检测到一种病原体，24 份标本中检测到两种或更多种病原体。总之，该多重 PCR 和 ACE 系统可从单个标本中快速、同时检测多种 STD 病原体，具有较高的灵敏度和特异性。
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