Khusaini Mohd Saif, Rahman Raja Noor Zaliha Raja Abd, Mohamad Ali Mohd Shukuri, Leow Thean Chor, Basri Mahiran, Salleh Abu Bakar
Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):401-3. doi: 10.1107/S1744309111002028. Epub 2011 Feb 25.
An organic solvent-tolerant lipase from Bacillus sp. strain 42 was crystallized using the capillary-tube method. The purpose of studying this enzyme was in order to better understand its folding and to characterize its properties in organic solvents. By initially solving its structure in the native state, further studies on protein-solvent interactions could be performed. X-ray data were collected at 2.0 Å resolution using an in-house diffractometer. The estimated crystal dimensions were 0.09×0.19×0.08 mm. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a=117.41, b=80.85, c=99.44 Å, β=96.40°.
使用毛细管法对芽孢杆菌属42号菌株的一种耐有机溶剂脂肪酶进行了结晶。研究这种酶的目的是为了更好地了解其折叠情况，并表征其在有机溶剂中的性质。通过首先解析其天然状态的结构，可以进一步开展蛋白质 - 溶剂相互作用的研究。使用内部衍射仪在2.0 Å分辨率下收集了X射线数据。估计晶体尺寸为0.09×0.19×0.08 mm。该晶体属于单斜空间群C2，晶胞参数为a = 117.41、b = 80.85、c = 99.44 Å，β = 96.40°。
