卵母细胞捐赠患者的胚胎通过时间 lapse 成像监测后的胚胎质量、囊胚和持续妊娠率。
Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging.
机构信息
Instituto Valenciano de Infertilidad, Alicante, Spain.
出版信息
J Assist Reprod Genet. 2011 Jul;28(7):569-73. doi: 10.1007/s10815-011-9549-1. Epub 2011 Mar 11.
Abstract
PURPOSE
In the current study, our aim was to demonstrate that EmbryoScope incubation conditions is comparable to standard laboratory incubation circumstances by comparing embryo quality, development and ongoing pregnancy rates between the EmbryoScope (ES) and a standard incubator (SI). We analyzed 478 embryos from 60 couples undergoing oocyte donation were included in the study.
METHODS
All embryos retrieved from a patient were randomly distributed in the ES or SI. We calculated blastocyst development rate, blastocyst viability and ongoing pregnancy rate for embryo transfers from ES, SI and mixed (one embryo from the ES and one from the SI). Statistical analysis was conducted by Chi square tests, considering p < 0.05 significant.
RESULTS
No significant differences were found between the ES and SI from all the parameters evaluated.
CONCLUSIONS
Thus we concluded that time-lapse monitoring in the EmbryoScope does not impair embryo quality while allowing for morphological, spatial and temporal analysis of embryo development.
摘要
目的
在本研究中，我们旨在通过比较胚胎培养箱（ES）和标准培养箱（SI）之间的胚胎质量、发育和持续妊娠率，来证明胚胎培养箱的孵育条件与标准实验室孵育条件相当。我们分析了 60 对接受卵母细胞捐赠的夫妇的 478 个胚胎。
方法
从患者中取出的所有胚胎均随机分配到 ES 或 SI 中。我们计算了 ES、SI 和混合（一个来自 ES，一个来自 SI）胚胎移植的囊胚发育率、囊胚活力和持续妊娠率。通过卡方检验进行统计学分析，认为 p < 0.05 有统计学意义。
结果
在评估的所有参数中，ES 和 SI 之间没有发现显著差异。
结论
因此，我们得出结论，胚胎培养箱中的时间 lapse 监测不会损害胚胎质量，同时允许对胚胎发育进行形态、空间和时间分析。
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Nat Biotechnol. 2010 Oct;28(10):1115-21. doi: 10.1038/nbt.1686. Epub 2010 Oct 3.
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Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial.使用冷冻保存的卵母细胞进行赠卵计划：一项前瞻性、随机、对照、临床试验。
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Evaluation of the safety of time-lapse observations for human embryos.评估人类胚胎延时观察的安全性。
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