School of Dentistry, Chung Shang Medical University, Taichung, Taiwan, R.O.C.
Phytother Res. 2011 Nov;25(11):1604-11. doi: 10.1002/ptr.3451. Epub 2011 Mar 11.
Although Rhodiola rosea (L.) is used widely and disseminated in Oriental medicine, its in vivo effects on cytokine modulation remain unclear. Among the biologically active components of Rhodiola rosea, salidroside was suggested to be the most active compound. The objectives of this study were to assess the toxicity and cytokine modulation effects of Rhodiola rosea standardised solution (RRSS) and salidroside. Quantitative high pressure liquid chromatography (HPLC) analysis determined the content of salidroside in RRSS to be 4.39% (w/v). Groups of Balb/c mice were fed daily with different doses of RRSS or salidroside, with CAPE or distilled water used as positive and negative controls, respectively. The acute and subacute toxicity tests did not reveal weight differences, pathological changes, or abnormalities in liver or kidney function indices among the treated groups. Ovalbumin-primed mouse cytokine assays demonstrated that both T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4 and IL-10) cytokines were significantly increased by feeding with RRSS in a dose- and time-dependent manner (p < 0.05). Moreover, the cytokine modulation effects of salidroside were less prominent than that of RRSS treatment and not dose-dependent. These findings suggest that increased secretion of both Th1- and Th2-pattern cytokines can be achieved with RRSS and salidroside treatment.
虽然红景天（L.）在东方医学中被广泛使用和传播，但它对细胞因子调节的体内作用仍不清楚。在红景天的生物活性成分中，红景天苷被认为是最活跃的化合物。本研究的目的是评估红景天标准化溶液（RRSS）和红景天苷的毒性和细胞因子调节作用。定量高效液相色谱（HPLC）分析确定 RRSS 中红景天苷的含量为 4.39%（w/v）。Balb/c 小鼠组每天用不同剂量的 RRSS 或红景天苷喂养，以 CAPE 或蒸馏水分别作为阳性和阴性对照。急性和亚急性毒性试验未显示出处理组之间的体重差异、组织病理学变化或肝肾功能指标异常。卵清蛋白诱导的小鼠细胞因子测定表明，RRSS 以剂量和时间依赖的方式显著增加了辅助性 T 细胞（Th1）（IL-2 和 IFN-γ）和 Th2（IL-4 和 IL-10）细胞因子（p<0.05）。此外，红景天苷的细胞因子调节作用不如 RRSS 治疗明显，也不是剂量依赖性的。这些发现表明，RRSS 和红景天苷治疗可导致 Th1 和 Th2 模式细胞因子的分泌增加。
