神经降压素降低人死后尾状核-壳核膜中多巴胺D2受体的亲和力。

Neurotensin reduces the affinity of dopamine D2 receptors in membranes from post mortem human caudate-putamen.

作者信息

von Euler G, Mailleux P, Vanderhaeghen J J, Fuxe K

机构信息

Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.

出版信息

Neurosci Lett. 1990 Feb 16;109(3):325-30. doi: 10.1016/0304-3940(90)90016-3.

DOI:10.1016/0304-3940(90)90016-3

PMID:2139501

Abstract

The effects of neurotensin in vitro were investigated on the binding characteristics of N-[3H]propylnorapomorphine [( 3H]NPA) binding sites in crude membrane preparations from post mortem human caudate-putamen, obtained within 10 h after death from 3 patients without reported neurological or psychiatric diseases. Neurotensin (0.1-30 nM) was found to increase the KD value of [3H]NPA binding sites with a maximal increase of about 50% (420 +/- 45 pM) at 3 nM of neurotensin, without significantly affecting the Bmax value (123 +/- 20 pmol/g protein). These results indicate the existence of an intramembrane regulation by neurotensin receptors of D2 receptors in the human caudate-putamen.

摘要

研究了神经降压素对死后人体尾状核 - 壳核粗膜制剂中N - [³H]丙基去甲阿朴吗啡（[³H]NPA）结合位点结合特性的体外作用。这些粗膜制剂取自3例无神经或精神疾病报告的患者，于死后10小时内获得。发现神经降压素（0.1 - 30 nM）可增加[³H]NPA结合位点的KD值，在3 nM神经降压素时最大增加约50%（420±45 pM），而对Bmax值（123±20 pmol/g蛋白质）无显著影响。这些结果表明在人体尾状核 - 壳核中存在神经降压素受体对D2受体的膜内调节作用。