Baxevanis C N, Sfagos C, Anastasopoulos E, Reclos G J, Papamichail M
Department of Immunology, Hellenic Anticancer Institute, Athens, Greece.
J Neuroimmunol. 1990 May;27(2-3):141-7. doi: 10.1016/0165-5728(90)90063-s.
Monocytes from patients with multiple sclerosis (MS) express decreased numbers of class II major histocompatibility complex (MHC) antigens in peripheral blood and are poor stimulators in the autologous mixed lymphocyte reaction (autoMLR). We assessed the effect of prothymosin-alpha (ProT alpha) on the expression of MHC class II antigens by monocytes. Immediately after isolation, monocytes were analyzed for MHC class II antigen expression using a radiolabelled monoclonal antibody specific for a monomorphic determinant on HLA-DR antigens. After incubation with ProT alpha we observed significant increases in HLA-DR antigens on MS monocytes (1.5- to 4-fold increase compared to freshly isolated monocytes). Kinetic analysis revealed that enhancement peaked after 2 days of incubation with ProT alpha. The increase in HLA-DR antigen on MS monocytes resulted in the restoration of the deficient autoMLR in MS patients. This is the first demonstration suggesting a link between HLA-DR antigen expression and cellular immune defects in MS. The significance of low autoMLR responses for T suppressor levels in MS patients is discussed.
多发性硬化症（MS）患者的单核细胞在外周血中表达的II类主要组织相容性复合体（MHC）抗原数量减少，并且在自体混合淋巴细胞反应（autoMLR）中是较弱的刺激物。我们评估了前胸腺素α（ProTα）对单核细胞MHC II类抗原表达的影响。分离后，立即使用针对HLA-DR抗原上单一决定簇的放射性标记单克隆抗体分析单核细胞的MHC II类抗原表达。与ProTα孵育后，我们观察到MS单核细胞上的HLA-DR抗原显著增加（与新鲜分离的单核细胞相比增加了1.5至4倍）。动力学分析显示，与ProTα孵育2天后增强达到峰值。MS单核细胞上HLA-DR抗原的增加导致MS患者缺陷性autoMLR的恢复。这是首次表明HLA-DR抗原表达与MS中的细胞免疫缺陷之间存在联系。讨论了MS患者中低autoMLR反应对T抑制水平的意义。
