Cardiocirculatory Unit, Research Center, Hospital Universitario La Fe, Valencia, Spain.
Int J Cardiol. 2012 Jul 12;158(2):199-204. doi: 10.1016/j.ijcard.2011.01.014. Epub 2011 Mar 10.
This study sought to determine pro-B-type natriuretic peptide (proBNP), BNP, N-terminal proBNP (NT-proBNP) and GATA-4 in the same cardiac tissue, the correlation among them, and the influence of ischemic etiology on their levels.
Protein levels were analyzed by Western blot techniques and mRNA expression was quantified by quantitative real-time polymerase chain reaction (RT-PCR) in a total of 33 human samples from ischemic (ICM), and control hearts.
Tissue protein level of proBNP is 1.5- and 12-fold higher than BNP or NT-proBNP respectively (p<0.0001), and BNP protein level was 8-fold higher than that of NT-proBNP (p<0.0001) in ICM hearts. Furthermore, proBNP mRNA expression was also increased in ICM (4-fold) compared to control hearts (p<0.05), but there was not a significant increase in GATA-4 mRNA. Then, tissue NP forms showed a high correlation among them (proBNP vs. BNP r=0.74, p<0.0001; proBNP vs. NT-proBNP r=0.43, p=0.03; and BNP vs. NT-proBNP r=0.61, p=0.001, respectively). Furthermore, GATA-4 with proBNP (r=0.536, p=0.007) and BNP (r=0.610, p=0.001) in ischemic samples. Finally, we found that proBNP, BNP, NT-proBNP and GATA-4 were increased in our ICM hearts (by 14%, p=0.004; 46%, p=0.024, 33%, p=0.002, and 49%, p=0.026, respectively) compared with controls.
This study shows higher protein level of proBNP in human hearts than of BNP and NT-proBNP, increased proBNP mRNA expression in ICM samples, and a good correlation among tissue natriuretic peptide and GATA-4. Finally, ICM shows a high tissue protein level of proBNP, BNP, NT-proBNP and GATA-4.
本研究旨在确定心脏组织中脑利钠肽前体（proBNP）、BNP、氨基末端脑利钠肽前体（NT-proBNP）和 GATA-4 的相关性，以及缺血性病因对它们水平的影响。
采用 Western blot 技术分析蛋白水平，采用实时定量聚合酶链反应（RT-PCR）定量 mRNA 表达，共分析了 33 例缺血性心肌病（ICM）和对照组心脏的组织样本。
ICM 心脏中，proBNP 蛋白水平分别比 BNP 和 NT-proBNP 高 1.5 倍和 12 倍（p<0.0001），BNP 蛋白水平比 NT-proBNP 高 8 倍（p<0.0001）。此外，与对照组相比，ICM 中 proBNP mRNA 表达也增加了（4 倍，p<0.05），但 GATA-4 mRNA 没有明显增加。然后，组织 NP 形式之间具有高度相关性（proBNP 与 BNP r=0.74，p<0.0001；proBNP 与 NT-proBNP r=0.43，p=0.03；BNP 与 NT-proBNP r=0.61，p=0.001）。此外，在缺血样本中，GATA-4 与 proBNP（r=0.536，p=0.007）和 BNP（r=0.610，p=0.001）相关。最后，我们发现与对照组相比，我们的 ICM 心脏中 proBNP、BNP、NT-proBNP 和 GATA-4 增加（增加 14%，p=0.004；增加 46%，p=0.024，增加 33%，p=0.002，增加 49%，p=0.026）。
本研究表明，人类心脏中 proBNP 的蛋白水平高于 BNP 和 NT-proBNP，ICM 样本中 proBNP mRNA 表达增加，组织利钠肽与 GATA-4 之间具有良好的相关性。最后，ICM 显示出高水平的 proBNP、BNP、NT-proBNP 和 GATA-4 组织蛋白。
