Department of Urology, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Urol Oncol. 2012 Nov-Dec;30(6):886-92. doi: 10.1016/j.urolonc.2010.08.017. Epub 2011 Mar 10.
To explore the expression profile of multiple cancer testis (CT) genes in transitional cell carcinoma of bladder (TCC), and investigate its possible correlation with clinicopathologic characteristics.
The mRNA expression of 6 CT genes was detected using reverse transcription polymerase chain reaction (RT-PCR) for 102 TCC samples (59 Ta-T1, 43 T2-T4, 44 G1, 32 G2, and 26 G3 samples) as well as the matching adjacent normal bladder mucosa for each sample. The MAGE-A3 protein expression was also determined by immunoblotting. Immunohistochemistry was performed in selected samples to confirm the MAGE-A3 protein expression.
The mRNA expression of all 6 CT genes was detected with relatively high frequencies in TCC tissues. The percent of samples positive for each gene in the TCC samples are: MAGE-A3, 58.8%; MAGE-A1, 56.9%; cTAGE-1, 52.9%; MAGE-A12, 51%; cTAGE-2, 49%; and NY-ESO-1, 45.1%. Furthermore, MAGE-A3 protein expression was positive in 52.9% of TCC tissues by immunoblotting. Immunohistochemistry showed an exclusively cytoplasmic staining pattern of MAGE-A3 protein. Neither CT gene mRNA expression nor MAGE-A3 protein expression was found in the adjacent normal tissue. There was no significant correlation between CT gene expression and clinicopathologic characteristics (P > 0.05).
All six CT genes are highly expressed in TCC, and may serve as therapeutic targets of specific immunotherapy for TCC, especially in multi-antigen vaccine preparations.
探讨多种癌症睾丸（CT）基因在膀胱移行细胞癌（TCC）中的表达谱，并研究其与临床病理特征的可能相关性。
采用逆转录聚合酶链反应（RT-PCR）检测 102 例 TCC 样本（59 例 Ta-T1、43 例 T2-T4、44 例 G1、32 例 G2 和 26 例 G3 样本）及相应匹配的相邻正常膀胱黏膜中 6 种 CT 基因的 mRNA 表达。采用免疫印迹法测定 MAGE-A3 蛋白的表达。在选定的样本中进行免疫组织化学检测以确认 MAGE-A3 蛋白的表达。
所有 6 种 CT 基因在 TCC 组织中均以较高频率检测到 mRNA 表达。TCC 样本中各基因阳性样本的百分比为：MAGE-A3，58.8%；MAGE-A1，56.9%；cTAGE-1，52.9%；MAGE-A12，51%；cTAGE-2，49%；NY-ESO-1，45.1%。此外，免疫印迹法显示 MAGE-A3 蛋白在 52.9%的 TCC 组织中呈阳性表达。免疫组织化学显示 MAGE-A3 蛋白呈独特的细胞质染色模式。相邻正常组织中均未检测到 CT 基因 mRNA 表达或 MAGE-A3 蛋白表达。CT 基因表达与临床病理特征之间无显著相关性（P＞0.05）。
六种 CT 基因在 TCC 中高度表达，可能成为 TCC 特异性免疫治疗的治疗靶点，尤其是在多抗原疫苗制备中。
