Department of Physical and Analytical Chemistry, University of Jaén, Spain.
Anal Chim Acta. 2011 Mar 18;689(2):226-33. doi: 10.1016/j.aca.2011.01.052. Epub 2011 Feb 3.
For the first time, a spectrofluorimetric method is reported for the simultaneous determination of resveratrol (RVT) and piceid (PCD), two stilbenes showing diverse interesting physiological and biochemical attributes, as well as a wide range of health benefits ranging from cardioprotection to chemoprevention. The method makes use of a multicommutated flow-through optosensor in which the resolution of RVT and PCD is accomplished by means the sequential arrival of their photoproducts, on-line generated by UV-irradiation, to the detection area. This is possible due to the different kinetic behaviour of these latter on a solid support (C(18) silica gel) filling a minicolumn placed before the detector. The measurement in solid-phase of the photochemically induced fluorescence of the photoproducts (λ(ex): 257 nm/λ(em): 382 nm) is used as analytical signal for monitoring both compounds. The method has been applied to the analysis of RVT and PCD in wines and requires a previous solid-phase extraction (SPE) using Bakerbond C(18) cartridges. This pretreatment and the use of a solid-support in both the minicolumn and the flow-cell of the detector allow the determination of RVT and PCD by external calibration. Detection limits (DLs) are 9.3 and 12.6 ng mL(-1) for RVT and PCD, respectively. Commercial red and white wine samples have been analysed and the results obtained have been satisfactorily validated by high-performance liquid chromatography (HPLC).
首次报道了一种同时测定白藜芦醇（RVT）和白皮杉醇（PCD）的荧光分光光度法。RVT 和 PCD 是两种具有不同有趣生理生化特性的芪类化合物，具有广泛的健康益处，从心脏保护到化学预防。该方法利用多交替流动式光传感器，通过连续到达其光产物的顺序，在线生成 UV 辐射，到达检测区域，实现 RVT 和 PCD 的分辨率。这是由于这些光产物在填充在检测器前的 minicolumn 中的固体载体（C（18）硅胶）上的不同动力学行为。光化学诱导荧光的固相测量（λ（ex）：257nm/λ（em）：382nm）用作监测两种化合物的分析信号。该方法已应用于葡萄酒中 RVT 和 PCD 的分析，需要使用 Bakerbond C（18）筒进行固相萃取（SPE）。这种预处理和在 minicolumn 和检测器的流动池两者中使用固体载体，允许通过外部校准来测定 RVT 和 PCD。RVT 和 PCD 的检测限（DL）分别为 9.3 和 12.6ngmL（-1）。分析了商业红葡萄酒和白葡萄酒样品，通过高效液相色谱（HPLC）对所得结果进行了满意的验证。
