Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobener Strasse, D-28359 Bremen, Germany.
J Microbiol Methods. 2011 Jun;85(3):228-9. doi: 10.1016/j.mimet.2011.03.002. Epub 2011 Mar 22.
A simple and practicable method for in-gel detection of bacterial produced L-amino acid oxidases (L-AAOs) after non-denaturing SDS-PAGE based on the visualisation of occurring hydrogen peroxide production is described. Advantages of this screening method for L-AAOs are the determination of their numbers and approximate molecular weights additionally in one approach.
本文描述了一种简单可行的方法,用于在非变性 SDS-PAGE 凝胶后检测细菌产生的 L-氨基酸氧化酶(L-AAOs),该方法基于对产生的过氧化氢的可视化。这种用于 L-AAOs 的筛选方法的优点在于可以在一种方法中同时确定它们的数量和近似分子量。