UMR IDES, Université Paris Sud, Orsay, France.
Comp Biochem Physiol B Biochem Mol Biol. 2011 Jul;159(3):131-9. doi: 10.1016/j.cbpb.2011.03.001. Epub 2011 Mar 22.
The nacre-prism transition of the mollusc shell Pinctada margaritifera was studied using scanning electron microscopy, electron probe micro-analysis (EPMA) and time-of-flight secondary ion mass spectrometry (TOF-SIMS). Mineralogical change is correlated with a change in organic matrix. Previous analyses had shown that sugars were involved in the transition layer (fibrous aragonite). The new Energy Dispersive Spectrometry (EDS) and TOF-SIMS maps show that the modifications at the layer boundary are complex, and that proteins and lipids are also involved. Detailed TOF-SIMS maps show that the thick organic envelopes surrounding the prisms, and between the prisms and the fibrous aragonitic layer, are not composed by regular layers, but are a patchwork of various molecules. The amino acid compositions of the nacreous and prismatic layer are compared thanks to the TOF-SIMS localized analyses.
采用扫描电子显微镜、电子探针微分析（EPMA）和飞行时间二次离子质谱（TOF-SIMS）研究了珍珠贝珍珠层的珍珠棱柱体转变。矿物变化与有机基质的变化相关。先前的分析表明，糖参与了过渡层（纤维文石）。新的能量色散光谱（EDS）和 TOF-SIMS 图谱表明，层边界的修饰很复杂，蛋白质和脂质也参与其中。详细的 TOF-SIMS 图谱表明，棱柱体周围以及棱柱体和纤维文石层之间的厚有机包膜不是由规则层组成，而是各种分子的拼凑。由于 TOF-SIMS 的局部分析，比较了珍珠层和棱柱层的氨基酸组成。
