pH对肌质网Ca2+ + Mg2(+)-激活的ATP酶活性的影响。
Effect of pH on the activity of the Ca2+ + Mg2(+)-activated ATPase of sarcoplasmic reticulum.
作者信息
Michelangeli F, Colyer J, East J M, Lee A G
机构信息
Department of Biochemistry, University of Southampton, U.K.
出版信息
Biochem J. 1990 Apr 15;267(2):423-9. doi: 10.1042/bj2670423.
Abstract
A kinetic model for the Ca2(+) + Mg2(+)-activated ATPase of sarcoplasmic reticulum was presented in a previous paper [Stefanova, Napier, East & Lee (1987) Biochem. J. 245, 723-730]. Here, that model is modified to account for the pH-dependence of ATPase activity and for the effects of Mg2+ on activity at high pH. It is shown that effects of Mg2+ on measurements of ATPase activity as a function of ATP concentration at pH 8.0 and pH 8.5 are consistent with binding of Mg2+ to the Ca2(+)-binding sites on the phosphorylated ATPase, such binding inhibiting dephosphorylation of the ATPase. It is also shown that slow dissociation of Ca2+ from the phosphorylated ATPase is consistent with the previously published model.
摘要
前一篇论文[斯特凡诺娃、内皮尔、伊斯特和李（1987年）《生物化学杂志》245卷，723 - 730页]提出了肌浆网Ca2(+) + Mg2(+)激活的ATP酶的动力学模型。在此，对该模型进行修改，以解释ATP酶活性的pH依赖性以及Mg2+在高pH下对活性的影响。结果表明，在pH 8.0和pH 8.5时，Mg2+对作为ATP浓度函数的ATP酶活性测量的影响与Mg2+结合到磷酸化ATP酶的Ca2(+)结合位点一致，这种结合抑制了ATP酶的去磷酸化。还表明，Ca2+从磷酸化ATP酶的缓慢解离与先前发表的模型一致。
相似文献
1
Effect of pH on the activity of the Ca2+ + Mg2(+)-activated ATPase of sarcoplasmic reticulum.pH对肌质网Ca2+ + Mg2(+)-激活的ATP酶活性的影响。
Biochem J. 1990 Apr 15;267(2):423-9. doi: 10.1042/bj2670423.
2
A kinetic model for Ca2+ efflux mediated by the Ca2+ + Mg2+-activated ATPase of sarcoplasmic reticulum.肌浆网Ca2+ + Mg2+激活的ATP酶介导的Ca2+外流动力学模型。
Biochem J. 1987 Aug 1;245(3):713-21. doi: 10.1042/bj2450713.
3
Effects of Mg2+, anions and cations on the Ca2+ + Mg2+-activated ATPase of sarcoplasmic reticulum.镁离子、阴离子和阳离子对肌浆网钙镁激活ATP酶的影响。
Biochem J. 1987 Aug 1;245(3):723-30. doi: 10.1042/bj2450723.
4
A kinetic model for the Ca2+ + Mg2+-activated ATPase of sarcoplasmic reticulum.肌浆网Ca2+ + Mg2+激活的ATP酶的动力学模型。
Biochem J. 1986 Jul 1;237(1):217-27. doi: 10.1042/bj2370217.
5
Binding of Ca2+ to the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum: kinetic studies.钙离子与肌浆网（钙-镁）-ATP酶的结合：动力学研究
Biochem J. 1994 Feb 1;297 ( Pt 3)(Pt 3):625-36. doi: 10.1042/bj2970625.
6
Rapid filtration study of the phosphorylation-dependent dissociation of calcium from transport sites of purified sarcoplasmic reticulum ATPase and ATP modulation of the catalytic cycle.纯化的肌浆网ATP酶转运位点上钙的磷酸化依赖性解离的快速过滤研究以及催化循环的ATP调节。
Biochemistry. 1986 Nov 18;25(23):7623-33. doi: 10.1021/bi00371a053.
7
A fast passive Ca2+ efflux mediated by the (Ca2+ + Mg2+)-ATPase in reconstituted vesicles.在重组囊泡中由（Ca2+ + Mg2+）-ATP酶介导的快速被动Ca2+外流。
Biochim Biophys Acta. 1987 Nov 2;904(1):45-54. doi: 10.1016/0005-2736(87)90085-x.
8
Labeling the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum with 4-(bromomethyl)-6,7-dimethoxycoumarin: detection of conformational changes.用4-(溴甲基)-6,7-二甲氧基香豆素标记肌浆网的（钙-镁）-ATP酶：构象变化的检测
Biochemistry. 1992 Jul 7;31(26):6023-31. doi: 10.1021/bi00141a010.
9
Mechanism of inhibition of the calcium pump of sarcoplasmic reticulum by thapsigargin.毒胡萝卜素抑制肌浆网钙泵的机制
Biochem J. 1992 Apr 15;283 ( Pt 2)(Pt 2):525-9. doi: 10.1042/bj2830525.
10
Modulation of the sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase by pentobarbital.戊巴比妥对肌浆网（Ca2+ + Mg2+）-ATP酶的调节作用。
Biochim Biophys Acta. 1990 Feb 16;1022(1):33-40. doi: 10.1016/0005-2736(90)90397-7.
引用本文的文献
1
Neferine induces autophagy-dependent cell death in apoptosis-resistant cancers via ryanodine receptor and Ca-dependent mechanism.荷叶碱通过肌浆网钙通道和钙依赖机制诱导凋亡抵抗性肿瘤细胞发生自噬依赖性细胞死亡。
Sci Rep. 2019 Dec 27;9(1):20034. doi: 10.1038/s41598-019-56675-6.
2
Ca signalling plays a role in celastrol-mediated suppression of synovial fibroblasts of rheumatoid arthritis patients and experimental arthritis in rats.钙信号在 celastrol 介导的类风湿关节炎患者滑膜成纤维细胞和大鼠实验性关节炎的抑制中发挥作用。
Br J Pharmacol. 2019 Aug;176(16):2922-2944. doi: 10.1111/bph.14718. Epub 2019 Jul 4.
3
-Desmethyldauricine Induces Autophagic Cell Death in Apoptosis-Defective Cells via Ca Mobilization.去甲基千金藤素通过钙动员在凋亡缺陷细胞中诱导自噬性细胞死亡。
Front Pharmacol. 2017 Jun 16;8:388. doi: 10.3389/fphar.2017.00388. eCollection 2017.
4
5
Saikosaponin-d, a novel SERCA inhibitor, induces autophagic cell death in apoptosis-defective cells.柴胡皂苷 d，一种新型的 SERCA 抑制剂，可诱导凋亡缺陷细胞发生自噬性细胞死亡。
Cell Death Dis. 2013 Jul 11;4(7):e720. doi: 10.1038/cddis.2013.217.
6
The widely utilized brominated flame retardant tetrabromobisphenol A (TBBPA) is a potent inhibitor of the SERCA Ca2+ pump.广泛使用的溴化阻燃剂四溴双酚A（TBBPA）是肌浆网Ca2+ 泵的一种强效抑制剂。
Biochem J. 2007 Dec 15;408(3):407-15. doi: 10.1042/BJ20070843.
7
The inhibition of the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase by macrocyclic lactones and cyclosporin A.大环内酯类药物和环孢素A对肌浆网/内质网Ca2+ -ATP酶的抑制作用
Biochem J. 2002 Aug 15;366(Pt 1):255-63. doi: 10.1042/BJ20020431.
8
Mechanism of inhibition of Ca(2+)-ATPase by myotoxin a.肌毒素a对Ca(2+)-ATP酶的抑制机制。
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):571-9. doi: 10.1042/bj3070571.
9
Identification, kinetic properties and intracellular localization of the (Ca(2+)-Mg2+)-ATPase from the intracellular stores of chicken cerebellum.鸡小脑细胞内储存库中（Ca²⁺-Mg²⁺）-ATP酶的鉴定、动力学特性及细胞内定位
Biochem J. 1991 May 1;275 ( Pt 3)(Pt 3):555-61. doi: 10.1042/bj2750555.
本文引用的文献
1
Occlusion of divalent cations in the phosphorylated calcium pump of sarcoplasmic reticulum.肌浆网磷酸化钙泵中二价阳离子的封闭
Eur J Biochem. 1980 Aug;109(1):231-8. doi: 10.1111/j.1432-1033.1980.tb04788.x.
2
Calcium and proton dependence of sarcoplasmic reticulum ATPase.肌浆网ATP酶对钙和质子的依赖性
Biophys J. 1983 Nov;44(2):271-80. doi: 10.1016/S0006-3495(83)84299-4.
3
Adenosine 5'-triphosphate at the active site accelerates binding of calcium to calcium adenosinetriphosphatase.活性位点处的三磷酸腺苷（ATP）可加速钙与钙-三磷酸腺苷酶的结合。
Biochemistry. 1984 Nov 6;23(23):5389-92. doi: 10.1021/bi00318a002.
4
5
Energetics of the calcium-transporting ATPase.钙转运ATP酶的能量学
J Biol Chem. 1984 Feb 10;259(3):1629-43.
6
Interdependence of H+, Ca2+, and Pi (or vanadate) sites in sarcoplasmic reticulum ATPase.肌浆网ATP酶中H⁺、Ca²⁺和磷酸根（或钒酸盐）位点的相互依赖性。
J Biol Chem. 1984 Jan 25;259(2):996-1003.
7
8
Low-temperature studies of the sarcoplasmic reticulum calcium pump. Mechanisms of calcium binding.肌浆网钙泵的低温研究。钙结合机制。
Biochim Biophys Acta. 1982 May 21;688(1):75-87. doi: 10.1016/0005-2736(82)90580-6.
9
The interaction of vanadate ions with the Ca-ATPase from sarcoplasmic reticulum.钒酸盐离子与肌浆网钙ATP酶的相互作用。
J Biol Chem. 1982 Jun 10;257(11):6111-9.
10
Binding of hydrophobic drugs to lipid bilayers and to the (Ca2+ + Mg2+)-ATPase.疏水性药物与脂质双层以及（Ca2+ + Mg2+）-ATP酶的结合。
Biochim Biophys Acta. 1983 Jul 27;732(2):428-40. doi: 10.1016/0005-2736(83)90060-3.
Zotero 插件