与粗糙脉孢菌中多胺生物合成改变相关的基因

Genes responsive to the alteration of polyamine biosynthesis in neurospora crassa.

作者信息

Grotewold E, Aisemberg G O, Taccioli G E, Judewicz N D

机构信息

Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina.

出版信息

Cell Biol Int Rep. 1990 Jan;14(1):69-78. doi: 10.1016/0309-1651(90)90072-7.

DOI:10.1016/0309-1651(90)90072-7

PMID:2139806

Abstract

Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment.

摘要

将生长在基本培养基中的野生型粗糙脉孢菌暴露于鸟氨酸脱羧酶（ODC-ase）活性的特异性抑制剂——α-二氟甲基鸟氨酸（DFMO）中。添加亚精胺可恢复受DFMO损害的蛋白质合成速率。在没有外源多胺的情况下，DFMO作用后，SDS-聚丙烯酰胺凝胶上的图谱显示出三种新合成的多肽，即p27、p31和p99。在添加亚精胺的培养基中生长的ODC-ase突变体（spe-1）未显示出诱导的多肽图谱。ODC-ase活性的缺乏促进了两种菌株中p27和p31编码基因的表达，但添加亚精胺后p31基因的转录被关闭。暴露于低剂量环己酰亚胺或营养饥饿后，这两种转录本也会积累。另一个编码p70的环己酰亚胺诱导基因在DFMO处理下也会表达。