多重 PCR 检测获得性碳青霉烯酶基因。
Multiplex PCR for detection of acquired carbapenemase genes.
机构信息
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France.
出版信息
Diagn Microbiol Infect Dis. 2011 May;70(1):119-23. doi: 10.1016/j.diagmicrobio.2010.12.002. Epub 2011 Mar 12.
A rapid and reliable PCR-based technique was developed for detection of genes encoding carbapenemases belonging to different classes. Primers were designed to amplify the following 11 genes: bla(IMP), bla(VIM), bla(NDM), bla(SPM), bla(AIM), bla(DIM), bla(GIM), bla(SIM)bla(KPC), bla(BIC), and bla(OXA-48). Three different multiplex reaction mixtures were defined and evaluated for the detection of all these 11 genes. Using optimized conditions, each reaction mixture allowed to identify the respective genes, with PCR giving distinct amplicon sizes corresponding to the different genes for each mixture. We reported here a rapid and reliable technique for screening all clinically relevant carbapenemase genes.
一种快速可靠的基于 PCR 的技术被开发出来，用于检测属于不同类别的编码碳青霉烯酶的基因。设计了引物来扩增以下 11 个基因：bla(IMP)、bla(VIM)、bla(NDM)、bla(SPM)、bla(AIM)、bla(DIM)、bla(GIM)、bla(SIM)bla(KPC)、bla(BIC)和 bla(OXA-48)。定义并评估了三种不同的多重反应混合物，以检测所有这 11 个基因。使用优化的条件，每个反应混合物都允许识别相应的基因，PCR 给出了与每种混合物中不同基因相对应的不同扩增子大小。我们在这里报告了一种快速可靠的技术，用于筛选所有临床相关的碳青霉烯酶基因。
Zotero 插件