通过 Rho 相关激酶（ROCK）抑制剂滴眼剂增强角膜内皮细胞的伤口愈合。

Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops.

机构信息

Department of Biomedical Engineering, Faculty of Life and Medical Sciences, Doshisha University, Tatara, Kyotanabe 610-0321, Japan.

出版信息

Br J Ophthalmol. 2011 Jul;95(7):1006-9. doi: 10.1136/bjo.2010.194571. Epub 2011 Mar 11.

DOI:10.1136/bjo.2010.194571

PMID:21398412

Abstract

AIM

To demonstrate the efficacy of Rho-associated kinase (ROCK) inhibitor Y-27632 for corneal endothelial wound healing both in in vitro and in vivo models.

METHODS

As an in vitro model, cultivated cynomolgus monkey corneal endothelial cells were scraped to create a linear defect. The wound distance was then determined during a 24-h culture in the presence or absence of 10 μM of Y-27632. As an in vivo model, central corneal endothelium of Japanese white rabbits was damaged by transcorneal freezing, then 10 mM of Y-27632 was applied topically six times daily for 48 h. The wound area of the corneal endothelium was evaluated after 48 h.

RESULTS

The mean wound distance in the cultured corneal endothelial cells was significantly shorter in the Y-27632 group than in the control group. In the rabbit model, the mean wound area of the Y-27632 group was significantly smaller than that of the control group.

CONCLUSION

This study demonstrated that ROCK inhibitor Y-27632 promotes corneal endothelial wound healing both in in vitro and in vivo.

摘要

目的

证明 Rho 相关激酶（ROCK）抑制剂 Y-27632 对体外和体内模型中角膜内皮伤口愈合的疗效。

方法

作为体外模型，刮取培养的食蟹猴角膜内皮细胞以产生线性缺陷。然后，在存在或不存在 10 μM 的 Y-27632 的情况下，在 24 小时的培养过程中确定伤口距离。作为体内模型，通过角膜冷冻损伤日本白兔的中央角膜内皮，然后每天局部应用 10 mM 的 Y-27632 六次，持续 48 小时。在 48 小时后评估角膜内皮的伤口面积。