Cons B M, Fox K R
Dept. Physiology & Pharmacology, University of Southampton, UK.
FEBS Lett. 1990 May 7;264(1):100-4. doi: 10.1016/0014-5793(90)80775-e.
DNA fragments containing (AT)n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT)n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A-DNA.
通过在药物存在的情况下使用多种核酸酶进行足迹实验，对含有克隆于假定光神霉素结合位点附近的(AT)n插入片段的DNA片段进行了检测。结果表明，光神霉素诱导了一种DNA结构变化，使相邻的(AT)n序列对DNase II的攻击敏感。DNase I和微球菌核酸酶也显示出显著变化。这些结果与一个模型一致，即光神霉素打开DNA小沟，将其改变为局部更类似于A-DNA的结构。
