台湾乌脚病疫区井水现存荧光腐殖质对体外凝血酶原时间和活化部分凝血活酶时间的影响

The effect of fluorescent humic substances existing in the well water of Blackfoot disease endemic areas in Taiwan on prothrombin time and activated partial thromboplastin time in vitro.

作者信息

Lu F J, Shih S R, Liu T M, Shown S H

机构信息

Department of Biochemistry, College of Medicine, National Taiwan University, Republic of China.

出版信息

Thromb Res. 1990 Mar 1;57(5):747-53. doi: 10.1016/0049-3848(90)90032-8.

DOI:10.1016/0049-3848(90)90032-8

PMID:2140208

Abstract

Fluorescent humic substances (FHS) in well water of Blackfoot disease endemic areas were purified and fractionated by Sephadex G-25 column chromatography. Four fractions of the purified FHS were isolated. The purified FHS and their fractions were then added to normal human pool plasma in vitro separately to detect the abilities of the effects on prothrombin time (PT) and activated thromboplastin time (APTT). Results showed that all of the four fractions of the purified FHS prolonged both PT and APTT in the higher concentration ranges (10 mg/ml - 20 mg/ml), but shortened both PT and APTT in the lower concentration ranges (0.5 mg/ml - 5 mg/ml). Among the four fractions of the FHS, the fraction 1, the humic substance with the highest molecular weight among all the four FHS, showed the most obvious effects. Owing to the effects of the FHS on PT and APTT values, we supposed that there is a close relationship between the FHS and the cause of Blackfoot disease.

摘要

对黑脚病流行地区井水的荧光腐殖质（FHS）进行了纯化，并通过葡聚糖G - 25柱色谱法进行分离。分离出了纯化FHS的四个组分。然后将纯化的FHS及其组分分别体外添加到正常人混合血浆中，以检测其对凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）的影响能力。结果显示，纯化FHS的所有四个组分在较高浓度范围（10 mg/ml - 20 mg/ml）均延长了PT和APTT，但在较低浓度范围（0.5 mg/ml - 5 mg/ml）均缩短了PT和APTT。在FHS的四个组分中，组分1，即所有四个FHS中分子量最高的腐殖质，显示出最明显的效果。鉴于FHS对PT和APTT值的影响，我们推测FHS与黑脚病的病因之间存在密切关系。