Sharma R, Woldehiwet Z, Spiller D G, Warenius H M
Department of Veterinary Pathology, University of Liverpool, Neston, Wirral, Great Britain.
Vet Immunol Immunopathol. 1990 Apr;24(4):383-91. doi: 10.1016/0165-2427(90)90008-g.
The lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (RSV) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. Experimental infection with bovine RSV was characterized by a significant rise in SBU-T8+ (CD8+ or cytotoxic) T cells and a significant reduction in SBU-T4+ (CD4+ or helper) T cells and B (LCA p220+) lymphocytes (P less than 0.05). The helper/suppressor (CD4/CD8) ratio was reduced from 3.91 on the day of experimental infection to 1.13 on 10 days after experimental infection (P less than 0.001). The total number of SBU-T4+ (CD4+) and B cells returned to pre-inoculation values 14 days after experimental infection but the helper/suppressor ratio remained depressed up to 21 days post-inoculation.
利用一组针对特定淋巴细胞表位的单克隆抗体，通过流式细胞术分析了正常羔羊以及实验感染牛呼吸道合胞病毒（RSV）的羔羊外周血中的淋巴细胞亚群。牛RSV实验感染的特征为SBU-T8+（CD8+或细胞毒性）T细胞显著增加，SBU-T4+（CD4+或辅助性）T细胞和B（LCA p220+）淋巴细胞显著减少（P<0.05）。辅助/抑制（CD4/CD8）比值从实验感染当天的3.91降至实验感染后10天的1.13（P<0.001）。实验感染后14天，SBU-T4+（CD4+）和B细胞总数恢复到接种前水平，但辅助/抑制比值在接种后21天仍保持较低水平。
