实验性感染牛呼吸道合胞病毒的羔羊体内的细胞毒性T细胞反应

Cytotoxic T cell responses in lambs experimentally infected with bovine respiratory syncytial virus.

作者信息

Sharma R, Woldehiwet Z

机构信息

University of Liverpool, Department of Veterinary Pathology, Neston, Wirral, UK.

出版信息

Vet Immunol Immunopathol. 1991 Jul;28(3-4):237-46. doi: 10.1016/0165-2427(91)90117-u.

DOI:10.1016/0165-2427(91)90117-u

PMID:1949569

Abstract

The role of cell-mediated immune response in the immunopathogenesis of bovine respiratory syncytial virus (BRSV) infection is not well established. In the present study, cytotoxic T cell responses of BRSV-infected lambs were examined using the chromium release assay. Lambs experimentally infected with BRSV developed cytotoxic lymphocytes in the peripheral blood and the spleen, which lysed BRSV-infected but not uninfected cells. Peak cytotoxic activity occurred 10-14 days after infection. Pretreatment of mononuclear cells with anti-CD8 monoclonal antibodies and rabbit complement significantly reduced cytotoxic activity (P less than 0.05). It appears, therefore, that lambs experimentally infected with BRSV develop virus-specific, predominantly CD8+, cytotoxic lymphocytes in the peripheral blood and spleen.

摘要

细胞介导的免疫反应在牛呼吸道合胞病毒（BRSV）感染的免疫发病机制中的作用尚未完全明确。在本研究中，使用铬释放试验检测了BRSV感染羔羊的细胞毒性T细胞反应。实验感染BRSV的羔羊在外周血和脾脏中产生了细胞毒性淋巴细胞，这些细胞毒性淋巴细胞可裂解被BRSV感染但未被感染的细胞。感染后10 - 14天出现细胞毒性活性峰值。用抗CD8单克隆抗体和兔补体预处理单核细胞可显著降低细胞毒性活性（P小于0.05）。因此，似乎实验感染BRSV的羔羊在外周血和脾脏中产生了病毒特异性的、主要为CD8 +的细胞毒性淋巴细胞。