Riske F, Chizzonite R, Nunes P, Stern A S
Department of Molecular Genetics, Roche Research Center, Hoffmann-La Roche Inc., Nutley, New Jersey.
Anal Biochem. 1990 Mar;185(2):206-12. doi: 10.1016/0003-2697(90)90281-d.
A soluble receptor binding assay has been developed for measuring human interleukin-1 alpha (IL-1 alpha), human IL-1 beta, and mouse IL-1 alpha. The assay is based on a competition between unlabeled IL-1 and 125I-labeled mouse recombinant IL-1 alpha for binding to soluble IL-1 receptor prepared from mouse EL-4 cells. The assay measures only biologically active IL-1 folded in its native conformation. The ratio of human IL-1 alpha to human IL-1 beta can be measured in the same sample by a pretreatment step which removes human IL-1 beta from samples prior to assay. This technique has been used to monitor the purification of recombinant IL-1, and may be utilized to specifically and accurately measure bioactive IL-1 in human serum and cell culture supernatants.
已开发出一种可溶性受体结合测定法，用于测量人白细胞介素-1α（IL-1α）、人IL-1β和小鼠IL-1α。该测定法基于未标记的IL-1与125I标记的小鼠重组IL-1α竞争结合从小鼠EL-4细胞制备的可溶性IL-1受体。该测定法仅测量以天然构象折叠的具有生物活性的IL-1。通过在测定前从样品中去除人IL-1β的预处理步骤，可以在同一样品中测量人IL-1α与人IL-1β的比例。该技术已用于监测重组IL-1的纯化，并且可用于特异性和准确地测量人血清和细胞培养上清液中的生物活性IL-1。
