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基于细胞色素b基因的澜沧江老挝纹胸鮡遗传结构与变异

[Genetic structure and variation of Glyptothorax laosensis of the Lancang River based on cytochrome b].

作者信息

Jin Ju, Liu Ming-Dian, Yin Shuang-Yu, Wang Deng-Qiang, Liu Shao-Ping, Chen Da-Qing

机构信息

College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Yi Chuan. 2011 Mar;33(3):255-61. doi: 10.3724/sp.j.1005.2011.00255.

Abstract

The hillstream catfish, Glyptothorax laosensis, is endemic to basin of Lancang River (also known as Mekong River). Genetic variation was detected within and among 8 populations of G. laosensis in Lancang River based on mitochondrial cytochrome b sequences. A total 16 nucleotides sites were detected in 1 138 bp fragment, which defined 15 haplotypes from 129 sequences. The variation level was very low, with mean h=0.299 and π=0.00032, respectively, and no variation was detected in some populations. Hierarchical analysis of molecular variance (AMOVA) showed no significant genetic structuring among populations. The unimodel mismatch distribution of pairwise haplotype difference and the negatively selective neutrality test (Tajima's D=2.3696, P<0.02; Fu's Fs=20.975, P<0.05) suggested that a recent population expansion had occurred.

摘要

湄公河纹胸鮡(Glyptothorax laosensis)是澜沧江(又称湄公河)流域特有的鱼类。基于线粒体细胞色素b序列,对澜沧江8个湄公河纹胸鮡种群的种内和种间遗传变异进行了检测。在1138bp片段中共检测到16个核苷酸位点,从129条序列中定义了15个单倍型。变异水平非常低,平均单倍型多样性(h)=0.299,核苷酸多样性(π)=0.00032,且在一些种群中未检测到变异。分子方差分析(AMOVA)表明种群间没有显著的遗传结构。成对单倍型差异的单峰错配分布和负选择中性检验(Tajima's D=2.3696,P<0.02;Fu's Fs=20.975,P<0.05)表明最近发生了种群扩张。

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