Dawson Paul Anthony, Sim Pearl, Simmons David Gordon, Markovich Daniel
School of Biomedical Sciences, University of Queensland, Brisbane QLD, Australia.
J Reprod Dev. 2011 Sep;57(4):444-9. doi: 10.1262/jrd.10-173k. Epub 2011 Mar 8.
Sulfate is important for growth and development, and is supplied from mother to fetus throughout pregnancy. We used NaS1 sulfate transporter null (Nas1(-/-)) mice to investigate the role of NaS1 in maintaining sulfate homeostasis during pregnancy and to determine the physiological consequences of maternal hyposulfataemia on fetal, placental and postnatal growth. We show that maternal serum (≤0.5 mM), fetal serum (<0.1 mM) and amniotic fluid (≤0.5 mM) sulfate levels were significantly lower in pregnant Nas1(-/-) mice when compared with maternal serum (≍2.0 mM), fetal serum (≍1.5 mM) and amniotic fluid (≍1.7 mM) sulfate levels in pregnant Nas1(+/+) mice. After 12 days of pregnancy, fetal reabsorptions led to markedly reduced (by ≥50%) fetal numbers in Nas1(-/-) mice. Placental labyrinth and spongiotrophoblast layers were increased (by ≍140%) in pregnant Nas1(-/-) mice when compared to pregnant Nas1(+/+) mice. Birth weights of progeny from female Nas1(-/-) mice were increased (by ≍7%) when compared to progeny of Nas1(+/+) mice. These findings show that NaS1 is essential to maintain high maternal and fetal sulfate levels, which is important for maintaining pregnancy, placental development and normal birth weight.
硫酸盐对生长发育至关重要，在整个孕期由母体供应给胎儿。我们使用NaS1硫酸盐转运蛋白缺失（Nas1(-/-)）小鼠来研究NaS1在孕期维持硫酸盐稳态中的作用，并确定母体低硫酸盐血症对胎儿、胎盘及出生后生长的生理影响。我们发现，与怀孕的Nas1(+/+)小鼠的母体血清（约2.0 mM）、胎儿血清（约1.5 mM）和羊水（约1.7 mM）硫酸盐水平相比，怀孕的Nas1(-/-)小鼠的母体血清（≤0.5 mM）、胎儿血清（<0.1 mM）和羊水（≤0.5 mM）硫酸盐水平显著降低。怀孕12天后，胎儿吸收导致Nas1(-/-)小鼠的胎儿数量显著减少（减少≥50%）。与怀孕的Nas1(+/+)小鼠相比，怀孕的Nas1(-/-)小鼠的胎盘迷路和海绵滋养层增加（约140%）。与Nas1(+/+)小鼠的后代相比，雌性Nas1(-/-)小鼠的后代出生体重增加（约7%）。这些发现表明，NaS1对于维持母体和胎儿的高硫酸盐水平至关重要，而这对于维持妊娠、胎盘发育和正常出生体重很重要。
